Allen P M, Matsueda G R, Haber E, Unanue E R
J Immunol. 1985 Jul;135(1):368-73.
The determinants recognized by two I-Ak-restricted hen egg-white lysozyme-specific T cell hybridomas were differentiated with a series of truncated or substituted peptides. The 10mer 52-61 was the smallest peptide that was immunogenic for both T cells. This peptide differed by a single residue, Leu56, from the corresponding autologous lysozyme peptide, which was nonimmunogenic. The addition of amino acids to the amino terminus of 52-61 increased the immunogenicity of the peptides for 3A9 T cells and decreased the immunogenicity for 2A11 T cells. By deleting or diiodinating Tyr53, the resulting peptides were rendered totally nonimmunogenic. In contrast, the 3-NO2-Tyr derivative was fully immunogenic for the 3A9 cells but completely nonimmunogenic for the 2A11 cells. Thus, two different, but very similar, determinants were generated by the same HEL peptide and the I-Ak molecule.
通过一系列截短或取代的肽段区分了两种受I-Ak限制的、针对鸡蛋清溶菌酶特异性的T细胞杂交瘤所识别的决定簇。10肽52-61是对两种T细胞均具有免疫原性的最小肽段。该肽段与相应的自身溶菌酶肽段仅在一个残基Leu56上存在差异，而自身溶菌酶肽段无免疫原性。在52-61的氨基末端添加氨基酸增加了肽段对3A9 T细胞的免疫原性，同时降低了对2A11 T细胞的免疫原性。通过缺失或用二碘酪氨酸取代Tyr53，所得肽段完全丧失免疫原性。相反，3-硝基酪氨酸衍生物对3A9细胞具有完全免疫原性，但对2A11细胞完全无免疫原性。因此，相同的溶菌酶肽段和I-Ak分子产生了两种不同但非常相似的决定簇。
