Winter Dominic, Dehghani Alireza, Steen Hanno
Institute for Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, Bonn, Germany,
Methods Mol Biol. 2015;1295:259-73. doi: 10.1007/978-1-4939-2550-6_20.
Mass spectrometry-based proteomics is the method of choice for analyzing (sub-) proteomes from virtually any source; with cells grown in culture being the most frequently investigated samples. Using HeLa cells, we describe a strategy for the optimization of protocols for whole proteome analysis. We cover cell lysis, protein precipitation and digestion, comparing the results obtained using different parameters and offering various possibilities for the optimization of a proteomic workflow which can be used for virtually any type of animal cell grown in culture.
基于质谱的蛋白质组学是分析几乎任何来源的（亚）蛋白质组的首选方法；培养的细胞是最常研究的样本。我们以HeLa细胞为例，描述了一种优化全蛋白质组分析方案的策略。我们涵盖了细胞裂解、蛋白质沉淀和消化，比较了使用不同参数获得的结果，并提供了多种优化蛋白质组学工作流程的可能性，该流程可用于几乎任何类型的培养动物细胞。
