Wang Jia, Yao Wen, Zhu Dan, Xie Weibo, Zhang Qifa
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Elife. 2015 Mar 30;4:e04250. doi: 10.7554/eLife.03913.
We performed a genetic analysis of sRNA abundance in flag leaf from an immortalized F2 (IMF2) population in rice. We identified 53,613,739 unique sRNAs and 165,797 sRNA expression traits (s-traits). A total of 66,649 s-traits mapped 40,049 local-sQTLs and 30,809 distant-sQTLs. By defining 80,362 sRNA clusters, 22,263 sRNA cluster QTLs (scQTLs) were recovered for 20,249 of all the 50,139 sRNA cluster expression traits (sc-traits). The expression levels for most of s-traits from the same genes or the same sRNA clusters were slightly positively correlated. While genetic co-regulation between sRNAs from the same mother genes and between sRNAs and their mother genes was observed for a portion of the sRNAs, most of the sRNAs and their mother genes showed little co-regulation. Some sRNA biogenesis genes were located in distant-sQTL hotspots and showed correspondence with specific length classes of sRNAs suggesting their important roles in the regulation and biogenesis of the sRNAs.
我们对水稻永生化F2（IMF2）群体旗叶中的小RNA丰度进行了遗传分析。我们鉴定出53,613,739个独特的小RNA和165,797个小RNA表达性状（s-性状）。总共66,649个s-性状定位到40,049个本地小RNA数量性状位点（local-sQTL）和30,809个远距离小RNA数量性状位点（distant-sQTL）。通过定义80,362个小RNA簇，在所有50,139个小RNA簇表达性状（sc-性状）中的20,249个中鉴定出22,263个小RNA簇数量性状位点（scQTL）。来自相同基因或相同小RNA簇的大多数s-性状的表达水平呈轻微正相关。虽然观察到一部分小RNA与其母基因之间以及来自相同母基因的小RNA之间存在遗传共调控，但大多数小RNA及其母基因几乎没有共调控。一些小RNA生物合成基因位于远距离sQTL热点区域，并且与特定长度类别的小RNA相对应，表明它们在小RNA的调控和生物合成中起重要作用。
