Pan Xinglu, Dong Fengshou, Xu Jun, Liu Xingang, Chen Zenglong, Liu Na, Chen Xixi, Tao Yan, Zhang Hongjun, Zheng Yongquan
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.
Anal Bioanal Chem. 2015 May;407(14):4111-20. doi: 10.1007/s00216-015-8603-8. Epub 2015 Mar 31.
A reliable and sensitive isotope-labelled internal standard method for simultaneous determination of chlorantraniliprole and cyantraniliprole in fruits (apple and grape), vegetables (cucumber and tomato) and cereals (rice and wheat) using ultra-high-performance liquid chromatography-tandem mass spectrometry was developed. Isotope-labelled internal standards were effective in compensating for the loss in the pretreatment and overcoming the matrix effect. The analytes were extracted with acetonitrile and cleaned up with different kinds of sorbents. The determination of the target compounds was achieved in less than 4 min using a T3 column combined with an electrospray ionization source in positive mode. The overall average relative recoveries in all matrices at three spiking levels (10, 20 and 50 μg kg(-1)) ranged from 95.5 to 106.2 %, with all relative standard deviations being less than 14.4 % for all analytes. The limits of detection did not exceed 0.085 μg kg(-1) and the limits of quantification were below 0.28 μg kg(-1) in all matrices. The method was demonstrated to be convenient and accurate for the routine monitoring of chlorantraniliprole and cyantraniliprole in fruits, vegetables and cereals.
建立了一种可靠且灵敏的同位素标记内标法，采用超高效液相色谱-串联质谱法同时测定水果（苹果和葡萄）、蔬菜（黄瓜和番茄）及谷物（大米和小麦）中的氯虫苯甲酰胺和氰虫苯甲酰胺。同位素标记内标有效地补偿了预处理过程中的损失并克服了基质效应。分析物用乙腈提取，并用不同种类的吸附剂净化。使用T3柱结合正模式电喷雾电离源，在不到4分钟的时间内实现了目标化合物的测定。在三个加标水平（10、20和50μg kg⁻¹）下，所有基质中的总体平均相对回收率为95.5%至106.2%，所有分析物的相对标准偏差均小于14.4%。所有基质中的检测限均不超过0.085μg kg⁻¹，定量限均低于0.28μg kg⁻¹。该方法被证明对于水果、蔬菜和谷物中氯虫苯甲酰胺和氰虫苯甲酰胺的常规监测既方便又准确。
