Govardovskiĭ V I, Dumler I L
Neirofiziologiia. 1985;17(2):245-50.
Liposomes loaded with monospecific cGMP-phosphodiesterase (PDE) antibodies act like PDE inhibitors (methylxantins, papaverine) on the aspartate-isolated rod and cone receptor potentials of the isolated perfused rod retina. The response amplitude drastically increases, especially at low stimulus strength, and its time course slows down. Pure lipid liposomes with no antibodies, liposomes loaded with control immunoglobulins, as well as PDE antibodies without liposomes, have no such actions. The effects are readily reversible by wash-out, which implies the liposome + antibodies action without penetrating the cells. The increase in the response amplitude can be only partly explained by the measured increase in the extracellular resistance of the photoreceptor layer under liposome action. It is supposed that the specific adsorption of liposomes on the photoreceptor cell surface changes the function of the intracellular transmission mechanism or (and) the properties of ionic channels of the plasma membrane in yet unknown way.
负载单特异性环鸟苷酸磷酸二酯酶（PDE）抗体的脂质体，对分离灌注的视杆视网膜中由天冬氨酸诱发的视杆和视锥细胞感受器电位，表现出类似PDE抑制剂（甲基黄嘌呤、罂粟碱）的作用。反应幅度急剧增加，尤其是在低刺激强度下，并且其时间进程减慢。不含抗体的纯脂质体、负载对照免疫球蛋白的脂质体以及不含脂质体的PDE抗体，均无此类作用。通过冲洗，这些作用很容易逆转，这意味着脂质体 + 抗体的作用不会穿透细胞。反应幅度的增加，只能部分地通过在脂质体作用下测量到的光感受器层细胞外电阻的增加来解释。据推测，脂质体在光感受器细胞表面的特异性吸附，以一种尚不清楚的方式改变了细胞内传递机制的功能或（和）质膜离子通道的特性。
