Dobersen M J, Hammer J A, Noronha A B, MacIntosh T D, Trapp B D, Brady R O, Quarles R H
Neurochem Res. 1985 Apr;10(4):499-513. doi: 10.1007/BF00964654.
A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. By contrast, all strains of mice were responsive to human MAG. The monoclonal antibodies developed differ with respect to immunoglobulin type, their specificity for human and/or rat MAG, and their recognition of protein or carbohydrate epitopes in MAG. In general, the antibodies that react with the protein backbone recognize both rat and human MAG, whereas a large number of the monoclonal antibodies recognize a carbohydrate determinant in human MAG that is not in rat MAG. Immunocytochemical staining of adult human spinal cord with the monoclonal antibodies resulted in periaxonal staining of myelin sheaths similar to that produced by well-defined, rabbit, polyclonal anti-MAG serum. In addition, the antibodies recognizing carbohydrate determinants in human MAG strongly stained oligodendrocyte cytoplasm. These monoclonal antibodies will be of value for the further chemical and biological characterization of MAG.
制备了一组针对大鼠和人髓鞘相关糖蛋白（MAG）的小鼠单克隆抗体。正常小鼠对大鼠MAG无反应，仅在自身免疫性NZB小鼠中成功实现了用大鼠MAG进行免疫。相比之下，所有品系的小鼠对人MAG均有反应。所制备的单克隆抗体在免疫球蛋白类型、对人及/或大鼠MAG的特异性以及对MAG中蛋白质或碳水化合物表位的识别方面存在差异。一般来说，与蛋白质主链反应的抗体可识别大鼠和人MAG，而大量单克隆抗体识别的是人类MAG中大鼠MAG所没有的碳水化合物决定簇。用这些单克隆抗体对成人脊髓进行免疫细胞化学染色，结果显示髓鞘的轴周染色与明确的兔抗MAG多克隆血清所产生的染色相似。此外，识别人类MAG中碳水化合物决定簇的抗体对少突胶质细胞胞质有强烈染色。这些单克隆抗体将有助于对MAG进行进一步的化学和生物学特性鉴定。
