Polat Fikriye, Diler Songül Budak, Azazi İrfan, Öden Artun
Department of Primary Education, Elementary Sciences Education, Faculty of Education, Kocaeli University, Kocaeli, Turkey E-mail :
Asian Pac J Cancer Prev. 2015;16(6):2199-202. doi: 10.7314/apjcp.2015.16.6.2199.
The aim of the present study was to determine whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in development of bladder cancer in the Turkish population. The study was performed on 75 patients (64 men, 11 women) with bladder cancer and 143 healthy individuals (107 men, 36 women) with any kind of cancer history. Three eNOS gene polymorphisms (T-786C promoter region, G894T and intron 4 VNTR 4a/b) were determined with polymerase chain reaction and restriction fragment lenght polymorphism methods. In our study, GT and TT genotypes for eNOS G894T polymorphism were found to significantly vary among patients with bladder cancer and control group (OR: 0.185, CI: 0.078-0.439, p=0.0001 and OR: 0.324, CI: 0.106-0.990, p=0.026). Also, the frequency of the 894T allele was significantly higher in patients with bladder cancer (51%). No association was identified for eNOS T-786C and intron 4 VNTR 4a/b polymorphisms between patients with bladder cancer and control groups in our Turkish population.
本研究的目的是确定内皮型一氧化氮合酶（eNOS）基因多态性是否在土耳其人群膀胱癌的发生中起作用。该研究对75例膀胱癌患者（64例男性，11例女性）和143名无任何癌症病史的健康个体（107例男性，36例女性）进行。采用聚合酶链反应和限制性片段长度多态性方法确定了三种eNOS基因多态性（T-786C启动子区域、G894T和内含子4 VNTR 4a/b）。在我们的研究中，发现eNOS G894T多态性的GT和TT基因型在膀胱癌患者和对照组之间存在显著差异（OR：0.185，CI：0.078-0.439，p = 0.0001；OR：0.324，CI：0.106-0.990，p = 0.026）。此外，膀胱癌患者中894T等位基因的频率显著更高（51%）。在我们的土耳其人群中，未发现膀胱癌患者与对照组之间eNOS T-786C和内含子4 VNTR 4a/b多态性存在关联。
