Qi Ying, Fan Jinping, Huang Weijin, Zhao Chenyan, Wang Youchun, Kong Franklin T, Kong Wei, Jiang Chunlai
School of Life Science, Jinlin University, Changchun, People's Republic of China.
National Engineering Laboratory for AIDS Vaccine, Jilin University, Changchun, People's Republic of China.
Biotechnol Appl Biochem. 2016 May;63(3):362-70. doi: 10.1002/bab.1379. Epub 2015 Jul 6.
The hepatitis E virus (HEV) capsid antigen expressed in insect cell has been proposed as a candidate subunit vaccine for the prevention of hepatitis E. However, the expression and purification of HEV virus-like particles (VLPs) from insect cells have not been explored. We aimed to optimize the procedure to obtain HEV VLPs. In this study, two conformations of the HEV capsid proteins were expressed in insect cells, VLPs and non-VLPs, and they were purified separately. The physicochemical properties and the humoral immune responses induced by the two forms were analyzed and compared. We found that HEV VLPs were more immunogenic in mice than HEV non-VLPs. Therefore, we optimized the conditions that yielded high VLPs expression in insect cell cultures and developed an efficient purification method. The results suggest that the distinction and isolation of VLPs from non-VLPs are essential to generate a more immunogenic vaccine.
在昆虫细胞中表达的戊型肝炎病毒（HEV）衣壳抗原已被提议作为预防戊型肝炎的候选亚单位疫苗。然而，尚未探索从昆虫细胞中表达和纯化HEV病毒样颗粒（VLP）的方法。我们旨在优化获得HEV VLP的程序。在本研究中，在昆虫细胞中表达了两种构象的HEV衣壳蛋白，即VLP和非VLP，并分别进行了纯化。分析并比较了这两种形式诱导的理化性质和体液免疫反应。我们发现，HEV VLP在小鼠中比HEV非VLP具有更强的免疫原性。因此，我们优化了在昆虫细胞培养物中产生高VLP表达的条件，并开发了一种有效的纯化方法。结果表明，区分和分离VLP与非VLP对于生产更具免疫原性的疫苗至关重要。
