采用毛细管电泳和高效液相色谱法检测血红蛋白恒河猴弹簧型和血红蛋白Q-泰国型的复合杂合子。
Detection of compound heterozygous of hb constant spring and hb q-Thailand by capillary electrophoresis and high performance liquid chromatography.
作者信息
Pornprasert Sakorn, Punyamung Manoo
机构信息
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, 110 Intawaroros Road, Chiang Mai, 50200 Thailand.
出版信息
Indian J Hematol Blood Transfus. 2015 Jun;31(2):229-32. doi: 10.1007/s12288-014-0411-3. Epub 2014 Jun 13.
A capillary electrophoresis (CE) has proven to be superior to a high performance liquid chromatography (HPLC) in the detection of hemoglobin Constant Spring (Hb CS). Thus the aim of this study was to analyze the efficacy of CE and HPLC for the detection of Hb CS in samples with compound heterozygous of Hb CS and Hb Q-Thailand. Hemoglobin analysis was performed in blood samples of 2 patients with compound heterozygous of Hb CS and Hb Q-Thailand by using HPLC and CE. The HPLC chromatogram and CE electrophoregram of the two techniques were compared. Hb CS was not found on HPLC chromatogram while Hb QA2 (α2 (QT)δ2), a derivative of Hb Q-Thailand, was presented at the retention time of 4.70-4.80 min and it was close to the retention time of Hb CS. On CE electrophoregram, Hb CS was presented at zone 2 (Z2) and it was distinctly separated from Hb QA2 which was presented at Z1. Therefore, CE was more efficient to the HPLC for diagnosis of compound heterozygous of Hb CS and Hb Q-Thailand.
在检测血红蛋白恒河猴（Hb CS）方面，毛细管电泳（CE）已被证明优于高效液相色谱（HPLC）。因此，本研究的目的是分析CE和HPLC在检测Hb CS与Hb Q-泰国双重杂合子样本中Hb CS的效能。使用HPLC和CE对2例Hb CS与Hb Q-泰国双重杂合子患者的血样进行血红蛋白分析。比较了两种技术的HPLC色谱图和CE电泳图。在HPLC色谱图上未发现Hb CS，而Hb Q-泰国的衍生物Hb QA2（α2（QT）δ2）在保留时间4.70 - 4.80分钟出现，且接近Hb CS的保留时间。在CE电泳图上，Hb CS出现在2区（Z2），并与出现在Z1的Hb QA2明显分离。因此，在诊断Hb CS与Hb Q-泰国双重杂合子时，CE比HPLC更有效。
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