Hromadnikova Ilona, Kotlabova Katerina, Ondrackova Marketa, Pirkova Petra, Kestlerova Andrea, Novotna Veronika, Hympanova Lucie, Krofta Ladislav
1Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
2Institute for the Care of Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
DNA Cell Biol. 2015 Jun;34(6):437-57. doi: 10.1089/dna.2014.2687. Epub 2015 Mar 31.
To demonstrate that pregnancy-related complications are associated with alterations in placental microRNA expression. Gene expression of 15 C19MC microRNAs (miR-512-5p, miR-515-5p, miR-516-5p, miR-517-5p, miR-518b, miR-518f-5p, miR-519a, miR-519d, miR-519e-5p, miR-520a-5p, miR-520h, miR-524-5p, miR-525, miR-526a, and miR-526b) was assessed in placental tissues, compared between groups (21 gestational hypertension [GH], 63 preeclampsia, 36 fetal growth restriction [FGR], and 42 normal pregnancies), and correlated with the severity of the disease with respect to clinical signs, delivery date, and Doppler ultrasound parameters. The expression profile of microRNAs was different between pregnancy-related complications and controls. The downregulation of 4 of 15 (miR-517-5p, miR-519d, miR-520a-5p, and miR-525), 6 of 15 (miR-517-5p, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, and miR-525), and 11 of 15 (miR-515-5p, miR-517-5p, miR-518b, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, miR-520h, miR-524-5p, miR-525, and miR-526a) microRNAs was associated with GH, FGR, and preeclampsia, respectively. Sudden onset of severe preeclampsia requiring immediate termination of gestation and mild forms of preeclampsia (persisting for several weeks) were associated with similar microRNA expression profile (downregulation of miR-517-5p, miR-520a-5p, miR-524-5p, and miR-525). In addition, miR-519a was found to be associated with severe preeclampsia. The longer the pregnancy-related disorder lasted, the more extensive was the downregulation of microRNAs (miR-515-5p, miR-518b, miR-518f-5p, miR-519d, and miR-520h). The downregulation of some C19MC microRNAs is a common phenomenon shared between GH, preeclampsia, and FGR. On the other hand, some of the C19MC microRNAs are only downregulated just in preeclampsia.
为证明妊娠相关并发症与胎盘微小RNA表达改变有关。评估了15种C19MC微小RNA（miR - 512 - 5p、miR - 515 - 5p、miR - 516 - 5p、miR - 517 - 5p、miR - 518b、miR - 518f - 5p、miR - 519a、miR - 519d、miR - 519e - 5p、miR - 520a - 5p、miR - 520h、miR - 524 - 5p、miR - 525、miR - 526a和miR - 526b）在胎盘组织中的基因表达，在不同组（21例妊娠期高血压[GH]、63例先兆子痫、36例胎儿生长受限[FGR]和42例正常妊娠）之间进行比较，并将其与疾病严重程度在临床体征、分娩日期和多普勒超声参数方面进行关联分析。妊娠相关并发症组与对照组之间微小RNA的表达谱不同。15种中的4种（miR - 517 - 5p、miR - 519d、miR - 520a - 5p和miR - 525）、6种（miR - 517 - 5p、miR - 518f - 5p、miR - 519a、miR - 519d、miR - 520a - 5p和miR - 525）以及15种中的11种（miR - 515 - 5p、miR - 517 - 5p、miR - 518b、miR - 518f - 5p、miR - 519a、miR - 519d、miR - 520a - 5p、miR - 520h、miR - 524 - 5p、miR - 525和miR - 526a）微小RNA的下调分别与GH、FGR和先兆子痫相关。需要立即终止妊娠的重度先兆子痫的突然发作和轻度先兆子痫形式（持续数周）与相似的微小RNA表达谱（miR - 517 - 5p、miR - 520a - 5p、miR - 524 - 5p和miR - 525的下调）相关。此外，发现miR - 519a与重度先兆子痫相关。妊娠相关疾病持续时间越长，微小RNA（miR - 515 - 5p、miR - 518b、miR - 518f - 5p、miR - 519d和miR - 520h）的下调越广泛。一些C19MC微小RNA的下调是GH、先兆子痫和FGR共有的常见现象。另一方面，一些C19MC微小RNA仅在先兆子痫中下调。
