Rotaru Maria, Totoianu Ioan Gheorghe, Sin Anca Ileana, Gheucă Solovăstru Laura
Department of Dermatology, "Victor Papilian" Faculty of Medicine, "Lucian Blaga" University of Sibiu, University Emergency Hospital of Sibiu, Romania;
Rom J Morphol Embryol. 2015;56(1):63-9.
To analyze the morphological data of pilo-sebaceous units in hirsute women before and 12 months after the antiandrogen treatment with Cyproterone acetate (CPA) 100 mg÷day.
Fourteen female patients with idiopathic hirsutism that followed an antiandrogen treatment with CPA were biopsied from the androgen-dependent area of the chin before and 12 months after the treatment. Routine sections were stained with Hematoxylin-Eosin, Masson, Van Gieson, Sirius red and picric-indigocarmine, while additional sections were immunostained for S100 protein and vimentin. Electron microscopy was performed in two cases with Langerhans cell hyperplasia.
On biopsies-stained sections, an increased number of hair follicles, the deeper part of the epithelial sheath of the hair follicle with epithelial buds, hyperplasia of sebaceous glands, and no inflammatory infiltrate were noticed. Langerhans cells identified with S100 protein and vimentin were normal in terms of numbers and distribution. After the administration of the treatment, atrophy of the pilo-sebaceous units was visible in nine (64.2%) cases, while inflammatory infiltrate and cells included in the vacuoles of the basal layer of the epidermis became apparent. In six of the cases treated with antiandrogens, a marked hyperplasia of Langerhans cells was noticed. In conclusion, the benefit of antiandrogen treatments is supported by atrophy of the hair follicle and the sebaceous glands. The activation of Langerhans cells associated with inflammatory infiltrate in the dermis and hair follicles could be considered as a local consequence of the involution process of hair follicles after the administration of the treatment.
分析100mg/天醋酸环丙孕酮（CPA）抗雄激素治疗前及治疗12个月后多毛女性毛囊皮脂腺单位的形态学数据。
14例接受CPA抗雄激素治疗的特发性多毛女性患者，在治疗前及治疗12个月后，从下巴的雄激素依赖区进行活检。常规切片用苏木精-伊红、Masson、Van Gieson、天狼星红和苦味酸-靛胭脂染色，另外的切片进行S100蛋白和波形蛋白免疫染色。对2例朗格汉斯细胞增生的病例进行电子显微镜检查。
在活检染色切片上，可见毛囊数量增加、毛囊上皮鞘深部有上皮芽、皮脂腺增生且无炎性浸润。用S100蛋白和波形蛋白鉴定的朗格汉斯细胞在数量和分布上正常。治疗后，9例（64.2%）可见毛囊皮脂腺单位萎缩，同时炎性浸润和表皮基底层空泡内的细胞变得明显。在6例接受抗雄激素治疗的病例中，注意到朗格汉斯细胞明显增生。总之，毛囊和皮脂腺萎缩支持了抗雄激素治疗的益处。朗格汉斯细胞的激活与真皮和毛囊中的炎性浸润相关，可被认为是治疗后毛囊退化过程的局部后果。
