生物活性天然产物的简单“按需”生产。
Simple "on-demand" production of bioactive natural products.
作者信息
Bode Edna, Brachmann Alexander O, Kegler Carsten, Simsek Rukayye, Dauth Christina, Zhou Qiuqin, Kaiser Marcel, Klemmt Petra, Bode Helge B
机构信息
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany) http://www.bio.uni-frankfurt.de/48050101/ak-bode.
出版信息
Chembiochem. 2015 May 4;16(7):1115-9. doi: 10.1002/cbic.201500094. Epub 2015 Mar 31.
Exchange of the native promoter to the arabinose-inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the "on-demand" production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel "mevalagmapeptides" and the highly toxic xenorhabdins were identified by this approach.
在昆虫病原细菌中，将天然启动子替换为阿拉伯糖诱导型启动子PBAD，以沉默和/或激活参与天然产物生物合成的基因簇。这使得能够“按需”生产GameX肽、异氨基霉素和蓝色色素靛蓝。通过这种方法鉴定了新型“甲戊拉肽”和剧毒杀线虫菌素的基因簇。
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