用于绵羊和山羊口疮病毒简易可视化检测的环介导等温扩增（LAMP）检测方法的开发与比较评估
Development and comparative evaluation of loop mediated isothermal amplification (LAMP) assay for simple visual detection of orf virus in sheep and goats.
作者信息
Venkatesan G, Bhanuprakash V, Balamurugan V
机构信息
Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India.
Pox Virus Laboratory, Division of Virology, Indian Veterinary Research Institute, Nainital (Distt.), Mukteswar 263 138, Uttarakhand, India.
出版信息
Mol Cell Probes. 2015 Jun;29(3):193-5. doi: 10.1016/j.mcp.2015.03.006. Epub 2015 Mar 27.
A loop-mediated isothermal amplification (LAMP) assay targeting DNA Pol gene was optimized and evaluated for the rapid detection of orf virus in clinical samples. The LAMP assay was found to be specific and sensitive. The detection rate of LAMP (89.3%) was better than PCR (67.9%) and comparable to real-time PCR (91.1%) in clinical samples by gel electrophoresis and visual detection methods. This LAMP assay is simple and does not rely upon any special equipment and could be employed in clinical diagnosis and epidemiological survey of orf infection.
针对DNA聚合酶基因的环介导等温扩增（LAMP）检测方法进行了优化，并评估其用于临床样本中羊口疮病毒快速检测的效果。结果发现该LAMP检测方法具有特异性和灵敏性。通过凝胶电泳和视觉检测方法，在临床样本中，LAMP的检测率（89.3%）优于聚合酶链反应（PCR，67.9%），与实时荧光定量PCR（91.1%）相当。这种LAMP检测方法操作简单，不依赖任何特殊设备，可用于羊口疮感染的临床诊断和流行病学调查。
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