Wen Zheng-Yong, Liang Xu-Fang, He Shan, Li Ling, Shen Dan, Tao Ya-Xiong
College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
Comp Biochem Physiol B Biochem Mol Biol. 2015 Jul;185:24-33. doi: 10.1016/j.cbpb.2015.03.005. Epub 2015 Mar 28.
Uncoupling proteins (UCPs) are mitochondrial anion carrier proteins, which play important roles in several physiological processes, including thermogenesis, reactive oxygen species generation, growth, lipid metabolism and insulin secretion. Although the roles of UCPs are well understood in mammals, little is known in fish. To investigate the thermogenesis roles in Chinese perch (Siniperca chuatsi), we cloned the UCP1, 2 and 3. The UCP1 consisted of six exons and five introns, and the UCP2 consisted of eight exons and seven introns. The UCP1 was primarily expressed in liver, UCP2 was ubiquitously expressed, and UCP3 was primarily expressed in muscle. The mRNA levels of UCP1 and UCP2 in liver, and UCP3 in muscle were significantly increased after prolonged cold exposure, but did not change after prolonged heat exposure, suggesting that Chinese perch might have a mechanism of response to cold environment, but not to hot environment. The intestinal UCP1 mRNA level was significantly up-regulated after prolonged heat exposure, while the UCP2 mRNA level was significantly up-regulated after prolonged cold exposure, suggesting that the two paralogs might play different roles in intestine of Chinese perch. In addition, the phylogenetic analysis could shed new light on the evolutionary diversification of UCP gene family.
解偶联蛋白（UCPs）是线粒体阴离子载体蛋白，在包括产热、活性氧生成、生长、脂质代谢和胰岛素分泌在内的多个生理过程中发挥重要作用。尽管UCPs在哺乳动物中的作用已得到充分了解，但在鱼类中却知之甚少。为了研究中国鲈（Siniperca chuatsi）的产热作用，我们克隆了UCP1、UCP2和UCP3。UCP1由六个外显子和五个内含子组成，UCP2由八个外显子和七个内含子组成。UCP1主要在肝脏中表达，UCP2广泛表达，UCP3主要在肌肉中表达。长时间冷暴露后，肝脏中UCP1和UCP2以及肌肉中UCP3的mRNA水平显著升高，但长时间热暴露后没有变化，这表明中国鲈可能有一种对寒冷环境的反应机制，但对炎热环境没有反应。长时间热暴露后，肠道UCP1的mRNA水平显著上调，而长时间冷暴露后，UCP2的mRNA水平显著上调，这表明这两个旁系同源物在中国鲈肠道中可能发挥不同的作用。此外，系统发育分析可以为UCP基因家族的进化多样性提供新的线索。
