Madhukaran Shanmuga Priyaa, Kishore Uday, Jamil Kaiser, Choolani Mahesh, Lu Jinhua
Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Centre for Biotechnology and Bioinformatics, School of Life Sciences, Jawaharlal Nehru Institute for Advanced Studies, Secunderabad, Andhra Pradesh, India; Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom.
Centre for Infection, Immunity and Disease Mechanisms, College of Health and Life Sciences, Brunel University London, Uxbridge, United Kingdom.
Mol Immunol. 2015 Aug;66(2):197-207. doi: 10.1016/j.molimm.2015.03.001. Epub 2015 Mar 28.
Surfactant proteins SP-A and SP-D, and complement protein C1q are soluble innate immune pattern recognizing molecules. SP-A, SP-D and C1q have an overall similar structure composed of an N-terminal triple-helical collagen region that is followed by a trimeric globular domain. While SP-A and SP-D belong to the collectin family (collagen containing lectin), C1q is the first recognition subcomponent of the classical pathway of the complement system. Recently, SP-A, SP-D and C1q have been considered to play important roles in early and late pregnancy. However, their expression in early human decidua has not been examined. Here, we investigated whether SP-A, SP-D and C1q are expressed within first trimester decidua in humans and their expression is associated with trophoblasts and decidual stromal cells. Decidual samples from women undergoing elective vaginal termination of pregnancy during first trimester were obtained from 25 subjects. Immunohistochemical studies using anti-human SP-A, anti-human SP-D and anti-human C1q antibodies were performed on decidual tissue sections along with anti-vimentin and cytokeratin-7 antibodies to identify stromal cells and trophoblasts. The expression was also examined by immunostaining and PCR using decidual and stromal cells. C1q expression was significantly higher when compared to SP-A and SP-D in the first trimester human decidua. Double immunostaining revealed that all stromal cells and trophoblasts expressed SP-A, SP-D and C1q, while only few invasive trophoblasts expressed C1q. Thus, expression of SP-A, SP-D and C1q in human decidua during first trimester suggests potential role of SP-A, SP-D and C1q during the early stages of pregnancy including implantation, trophoblast invasion and placental development.
表面活性蛋白SP-A和SP-D以及补体蛋白C1q是可溶性先天性免疫模式识别分子。SP-A、SP-D和C1q具有总体相似的结构，由一个N端三螺旋胶原区域组成，其后是一个三聚体球状结构域。虽然SP-A和SP-D属于凝集素家族（含胶原的凝集素），但C1q是补体系统经典途径的首个识别亚成分。最近，SP-A、SP-D和C1q被认为在妊娠早期和晚期发挥重要作用。然而，它们在人早期蜕膜中的表达尚未得到研究。在此，我们研究了SP-A、SP-D和C1q是否在人类妊娠早期蜕膜中表达，以及它们的表达是否与滋养层细胞和蜕膜基质细胞相关。从25名受试者获取了妊娠早期接受选择性阴道终止妊娠的女性的蜕膜样本。使用抗人SP-A、抗人SP-D和抗人C1q抗体对蜕膜组织切片进行免疫组织化学研究，并同时使用抗波形蛋白和细胞角蛋白-7抗体来识别基质细胞和滋养层细胞。还通过对蜕膜和基质细胞进行免疫染色和PCR来检测表达情况。在妊娠早期人蜕膜中，C1q的表达与SP-A和SP-D相比显著更高。双重免疫染色显示，所有基质细胞和滋养层细胞均表达SP-A、SP-D和C1q，而只有少数侵入性滋养层细胞表达C1q。因此，SP-A、SP-D和C1q在妊娠早期人蜕膜中的表达表明它们在妊娠早期阶段包括着床、滋养层细胞侵袭和胎盘发育过程中具有潜在作用。
