Ghasemnezhad Mahmood, Zareh Somayeh, Shiri Mohammad Ali, Javdani Zahra
Department of Horticultural Science, Faculty of Agriculture, University of Guilan, Rasht, Iran.
J Food Sci Technol. 2015 Apr;52(4):2023-32. doi: 10.1007/s13197-013-1213-6. Epub 2013 Dec 3.
In this study, the quality of minimally processed different Iranian pomegranate genotypes was investigated during storage at 4 °C for 14 days. The results showed that at the end of storage time, the lowest microbial count was found on the arils of "Torsh Syabe Lorestan" genotype. There was a significant difference in titratable acidity, total soluble solids, total anthocyanin, catechin, and quercetin content in most genotypes after 14-day cold storage; while no difference was found between studied genotypes in antioxidant capacity, total phenolic, and flavonoid content. In general, total phenolic content and antioxidant capacity of pomegranate arils gradually decreased over storage time. Diphenolase activity of polyphenol oxidase (PPO) enzyme with dopamine hydrochloride substrate and peroxidase (POD) decreased over storage time, whereas diphenolase activity of PPO with pyrocatechol substrate significantly increased. The lowest diphenolase activity with dopamine hydrochloride and pyrocatechol substrates, as well as POD activity was found in Torsh Syabe Lorestan. The results suggested that the genotype of Torsh Syabe Lorestan, which showed the lowest microbial count on the arils and enzymes, could be more appropriate for minimal processing technology.
在本研究中，对不同伊朗石榴基因型的最低加工品在4℃下储存14天期间的品质进行了调查。结果表明，在储存期结束时，“Torsh Syabe Lorestan”基因型的石榴籽上微生物数量最低。在14天冷藏后，大多数基因型的可滴定酸度、总可溶性固形物、总花青素、儿茶素和槲皮素含量存在显著差异；而在所研究的基因型之间，抗氧化能力、总酚和类黄酮含量没有差异。总体而言，石榴籽的总酚含量和抗氧化能力随储存时间逐渐降低。以盐酸多巴胺为底物的多酚氧化酶（PPO）和过氧化物酶（POD）的双酚酶活性随储存时间降低，而以邻苯二酚为底物的PPO双酚酶活性显著增加。在Torsh Syabe Lorestan中发现以盐酸多巴胺和邻苯二酚为底物时双酚酶活性最低，以及POD活性最低。结果表明，Torsh Syabe Lorestan基因型在石榴籽和酶方面微生物数量最低，可能更适合最低加工技术。
