Yamashita S, Newbold R R, McLachlan J A, Korach K S
Receptor Biology Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Endocrinology. 1989 Dec;125(6):2888-96. doi: 10.1210/endo-125-6-2888.
The distribution of the estrogen receptor (ER) was investigated in neonatal female genital tracts (uterus, oviduct, cervix, and vagina) from days 1-22 after birth, using immunohistochemistry employing an anti-ER monoclonal antibody. In uteri, the ER in epithelial cells began to be observed by day 4. The number of positive epithelial cells and the staining intensity gradually increased until day 22 of age. On the other hand, uterine stroma cells gave a strong ER immunostaining even on day 1. The staining intensity reached a maximum by days 4-7 and then slightly decreased with age. In the oviduct, cervix, and vagina, epithelial cells showed positive ER immunostaining on day 1, and the intensity increased gradually until day 22. ER immunostaining in stroma cells was almost constant during the development period. The ER in both epithelial and stroma cells from these younger animals showed similar biochemical properties, i.e. an increased affinity for nuclei and resistance to extraction with PBS. Thus, during neonatal development of the female reproductive tract, ER is present not only in stroma cells but also in epithelial cells. This ER protein exhibits properties and characteristics similar to those of adult mice. The presence of ER suggests that some of the estrogen actions of cell proliferation, differentiation, and tissue abnormalities resulting from prenatal and postnatal estrogen administration may be mediated by receptor interactions.
采用抗雌激素受体（ER）单克隆抗体免疫组化法，研究出生后1至22天新生雌性生殖道（子宫、输卵管、宫颈和阴道）中ER的分布。在子宫中，上皮细胞中的ER在第4天开始被观察到。阳性上皮细胞数量和染色强度在22日龄前逐渐增加。另一方面，子宫基质细胞在第1天就呈现出强烈的ER免疫染色。染色强度在第4至7天达到最大值，然后随年龄略有下降。在输卵管、宫颈和阴道中，上皮细胞在第1天显示出阳性ER免疫染色，强度在22日龄前逐渐增加。基质细胞中的ER免疫染色在发育期间几乎保持不变。这些幼年动物上皮细胞和基质细胞中的ER显示出相似的生化特性，即对细胞核的亲和力增加以及对PBS提取具有抗性。因此，在雌性生殖道的新生儿发育过程中，ER不仅存在于基质细胞中，也存在于上皮细胞中。这种ER蛋白表现出与成年小鼠相似的特性和特征。ER的存在表明，产前和产后给予雌激素所导致的细胞增殖、分化和组织异常等一些雌激素作用可能是由受体相互作用介导的。
