Monpezat J P, Frindel E
Cellular Kinetics Research Unit, INSERM U.250, Gustave-Roussy Institute, Villejuif, France.
Exp Hematol. 1989 Dec;17(11):1077-80.
In order to further investigate the mechanisms of spleen colony-forming unit (CFU-S) inhibition by the tetrapeptide acetyl-N-Ser-Asp-Lys-Pro (AcSDKP), the following related subjects were studied: 1) the effects of AcSDKP on the kinetics of granulocyte and macrophage precursor cells (granulocyte-macrophage colony-forming cells; GM-CFC); 2) the precise point in the cell cycle of CFU-S that is sensitive to AcSDKP; and 3) the role of lymphocytes in the chain of events leading to the inhibition of CFU-S entry into the cell cycle. The effects of AcSDKP on CFU-S and GM-CFC were tested using the spleen colony assay and methylcellulose culture technique, respectively; the cell cycle kinetic status was determined by the tritiated thymidine suicide technique. Nude mice were studied to assess the role of T-lymphocytes in the inhibitory phenomenon. Our results indicate that: 1) there is no inhibitory effect of AcSDKP on GM-CFC in vitro or in vivo; 2) AcSDKP is active at only the G0 or early G1 phases; and 3) AcSDKP activity is not modulated by T cells.
为了进一步研究四肽乙酰 - N - 丝氨酸 - 天冬氨酸 - 赖氨酸 - 脯氨酸（AcSDKP）抑制脾集落形成单位（CFU - S）的机制，对以下相关课题进行了研究：1）AcSDKP对粒细胞和巨噬细胞前体细胞（粒细胞 - 巨噬细胞集落形成细胞；GM - CFC）动力学的影响；2）CFU - S细胞周期中对AcSDKP敏感的精确时间点；3）淋巴细胞在导致CFU - S进入细胞周期受抑制这一事件链中的作用。分别采用脾集落测定法和甲基纤维素培养技术检测AcSDKP对CFU - S和GM - CFC的影响；通过氚标记胸腺嘧啶核苷自杀技术确定细胞周期动力学状态。研究裸鼠以评估T淋巴细胞在抑制现象中的作用。我们的结果表明：1）AcSDKP在体外或体内对GM - CFC均无抑制作用；2）AcSDKP仅在G0期或G1早期具有活性；3）AcSDKP的活性不受T细胞调节。
