Robinson S, Lenfant M, Wdzieczak-Bakala J, Melville J, Riches A
School of Biological and Medical Sciences, University of St. Andrews, Scotland, UK.
Cell Prolif. 1992 Nov;25(6):623-32. doi: 10.1111/j.1365-2184.1992.tb01464.x.
The mechanism of action of the haemoregulatory tetrapeptide Acetyl-N-Ser-Asp-Lys-Pro (AcSDKP, M(r) = 487 amu), was investigated using an in vitro assay of a murine high proliferative potential colony-forming cell (HPP-CFC) which responds to proliferation regulators of the haematopoietic stem cell population. AcSDKP had no direct inhibitory effect on the number, or the proportion in S phase, of the committed granulocyte-macrophage progenitor (GM-CFC), or cycling HPP-CFC populations. However, AcSDKP blocked the action of a stimulator of haematopoietic stem cell proliferation, preventing the switching of quiescent HPP-CFC into cell cycle. It would appear that AcSDKP exerts its inhibitory haemoregulatory role indirectly, by preventing stimulator action on haematopoietic stem cells.
利用对造血干细胞群体增殖调节剂有反应的小鼠高增殖潜能集落形成细胞（HPP-CFC）的体外测定法，研究了血液调节四肽乙酰基-N-丝氨酸-天冬氨酸-赖氨酸-脯氨酸（AcSDKP，分子量=487原子质量单位）的作用机制。AcSDKP对定向粒细胞-巨噬细胞祖细胞（GM-CFC）或处于细胞周期的HPP-CFC群体的数量或S期比例没有直接抑制作用。然而，AcSDKP阻断了造血干细胞增殖刺激剂的作用，阻止静止的HPP-CFC进入细胞周期。看来AcSDKP通过阻止刺激剂对造血干细胞的作用，间接发挥其血液调节抑制作用。
