来自兔网织红细胞的翻译活性信使核糖核蛋白颗粒（mRNPs）在无细胞系统中的可翻译性与游离信使核糖核酸（mRNA）在性质上有所不同。

Translational active mRNPs from rabbit reticulocytes are qualitatively different from free mRNA in their translatability in cell-free system.

作者信息

Minich W B, Korneyeva N L, Ovchinnikov L P

机构信息

Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.

出版信息

FEBS Lett. 1989 Nov 6;257(2):257-9. doi: 10.1016/0014-5793(89)81547-9.

DOI:10.1016/0014-5793(89)81547-9

PMID:2583271

Abstract

The translatability of polyribosomal and free mRNPs from rabbit reticulocytes and their mRNA was compared. Both classes of mRNPs turned out to be active in rabbit reticulocyte lysates. Considerable differences between mRNPs and mRNA have been revealed. The most striking feature of mRNPs was that high concentrations of mRNPs do not inhibit protein biosynthesis, whereas high concentrations of mRNA strongly inhibit this process. This inhibition is specific for mRNA and does not occur at the addition of the same amount of rRNA from E. coli. The features of mRNP translation are not the result of addition of the supplementary translation factors within particles. The specific function of mRNP proteins in the process of translation is under discussion.

摘要

比较了来自兔网织红细胞的多核糖体和游离mRNA颗粒（mRNPs）的可翻译性及其mRNA。结果表明，这两类mRNA颗粒在兔网织红细胞裂解物中均具有活性。已揭示出mRNA颗粒与mRNA之间存在显著差异。mRNA颗粒最显著的特征是，高浓度的mRNA颗粒不会抑制蛋白质生物合成，而高浓度的mRNA则会强烈抑制这一过程。这种抑制作用对mRNA具有特异性，在添加等量来自大肠杆菌的rRNA时不会发生。mRNA颗粒翻译的特征并非颗粒内添加补充翻译因子的结果。目前正在讨论mRNA颗粒蛋白在翻译过程中的具体功能。