给硫代乙酰胺预处理的大鼠或部分肝切除的大鼠注射放线菌酮对分离的肝细胞核中RNA聚合酶I活性的影响。

Effect of the administration of cycloheximide to thioacetamide-primed rats or partial-hepatectomized rats on the RNA polymerase I activity in isolated nuclei of liver.

Suzuki N, Matsui H, Onodera M, Hosoya T

Effect of Pharmaceutical Sciences, Chiba University, Japan.

Int J Biochem. 1989;21(10):1127-31. doi: 10.1016/0020-711x(89)90054-2.

Abstract

Twenty-four hours after administration of thioacetamide to normal rats, the activity and amount of RNA polymerase I in isolated liver nuclei were almost doubled. 2. When cycloheximide was administered to the drug-treated rats and normal rats 1 or 2 hr before death, the reduction in the activity was of the same degree, that is, about half of the activity of liver nuclei from normal rats. 3. Partial hepatectomy also caused about 2-fold increase in the RNA polymerase I activity in isolated liver nuclei after 16 hr, but the increased activity was almost completely abolished by injection of cycloheximide 1-3 hr before killing.

摘要

给正常大鼠注射硫代乙酰胺24小时后，分离的肝细胞核中RNA聚合酶I的活性和数量几乎增加了一倍。2. 在处死前1或2小时给经药物处理的大鼠和正常大鼠注射环己酰亚胺，活性降低程度相同，即约为正常大鼠肝细胞核活性的一半。3. 部分肝切除术后16小时，分离的肝细胞核中RNA聚合酶I的活性也增加了约2倍，但在处死前1 - 3小时注射环己酰亚胺后，增加的活性几乎完全被消除。