人血中循环滤泡辅助性T细胞（Tfh）和滤泡调节性T细胞（Tfr）群体的流式细胞术分析。
Flow cytometric analysis of circulating follicular helper T (Tfh) and follicular regulatory T (Tfr) populations in human blood.
作者信息
Wei Yunbo, Feng Jinhong, Hou Zhaohua, Wang Xin Maggie, Yu Di
机构信息
Laboratory of Immunology for Environment and Health, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong, 250014, China.
出版信息
Methods Mol Biol. 2015;1291:199-207. doi: 10.1007/978-1-4939-2498-1_17.
Follicular helper T (Tfh) cells are the helper T-cell subset that localizes in germinal centers within secondary lymphoid organs. They support B cells to produce high-affinity antibodies and generate B-cell memory. By contrast, follicular regulatory T (Tfr) cells localize in germinal centers to suppress B-cell responses. Coordinately regulating antibody responses, Tfh and Tfr cells play a critical role for vaccination, infectious disease control, and the development of autoimmune diseases. Although bona fide Tfh and Tfr cells are hardly detected in human blood, circulating Tfh and Tfr memory cells in blood can be used to investigate their functions in health and disease. We have developed a comprehensive flow cytometric analysis to define different circulating Tfh and Tfr populations within CXCR5(+) CD4(+) T-cell population in human blood, based on numerous cell surface markers including CD25, CD127, CCR7, PD-1, CXCR3, and CCR6.
滤泡辅助性T（Tfh）细胞是定位在次级淋巴器官生发中心的辅助性T细胞亚群。它们支持B细胞产生高亲和力抗体并产生B细胞记忆。相比之下，滤泡调节性T（Tfr）细胞定位在生发中心以抑制B细胞反应。Tfh和Tfr细胞协同调节抗体反应，在疫苗接种、传染病控制和自身免疫性疾病的发展中起关键作用。虽然在人血液中很难检测到真正的Tfh和Tfr细胞，但血液中的循环Tfh和Tfr记忆细胞可用于研究它们在健康和疾病中的功能。我们基于包括CD25、CD127、CCR7、PD-1、CXCR3和CCR6在内的众多细胞表面标志物，开发了一种综合流式细胞术分析方法，以定义人血液中CXCR5(+) CD4(+) T细胞群体内不同的循环Tfh和Tfr亚群。
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