Shargill N S, Lupien J R, Bray G A
Section of Diabetes and Clinical Nutrition, School of Medicine, University of Southern California, Los Angeles.
Horm Metab Res. 1989 Sep;21(9):463-7. doi: 10.1055/s-2007-1009264.
Adrenalectomy (ADX) prevents the excessive weight gain in the genetically obese ob/ob and db/db mice. To test the possibility that this results from increased energy expenditure due to increased thermogenesis in brown adipose tissue (BAT), we measured GDP binding to mitochondria from interscapular brown adipose tissue (BAT) in db/db and ob/ob mice and their lean controls after adrenalectomy, with and without corticosterone replacement. Both the vehicle treated and corticosterone treated db/db and ob/ob mice had lower body weights than the sham-operated mice GDP binding to mitochondria from IBAT was significantly lower in both the db/db and ob/ob mice than in their lean controls. Adrenalectomy significantly increased GDP binding in all mice compared to the respective sham-operated mice, but, the percentage increase was always greater in the db/db and ob/ob mice. Corticosterone treatment of adrenalectomized db/db, ob/ob or lean mice lowered GDP binding to sham levels. Our data confirm previous findings that adrenalectomy results in increased GDP binding to mitochondria from IBAT. Injections of corticosterone into adrenalectomized mice results in a decrease in GDP binding to values which are similar to values in sham-operated mice. Thus adrenalectomy may inhibit the development of obesity by increasing the thermic activity in IBAT.
肾上腺切除术（ADX）可防止遗传性肥胖的ob/ob和db/db小鼠过度增重。为了验证这是否是由于棕色脂肪组织（BAT）产热增加导致能量消耗增加所致，我们测量了肾上腺切除术后给予或未给予皮质酮替代治疗的db/db和ob/ob小鼠及其瘦型对照小鼠肩胛间棕色脂肪组织（BAT）中线粒体的GDP结合情况。与假手术小鼠相比，给予载体处理和皮质酮处理的db/db和ob/ob小鼠体重均较低。db/db和ob/ob小鼠IBAT中线粒体的GDP结合均显著低于其瘦型对照。与各自的假手术小鼠相比，肾上腺切除术显著增加了所有小鼠的GDP结合，但db/db和ob/ob小鼠的增加百分比总是更大。对肾上腺切除的db/db、ob/ob或瘦型小鼠进行皮质酮治疗可使GDP结合降至假手术水平。我们的数据证实了先前的发现，即肾上腺切除术导致IBAT中线粒体的GDP结合增加。向肾上腺切除的小鼠注射皮质酮会导致GDP结合减少，其值与假手术小鼠的值相似。因此，肾上腺切除术可能通过增加IBAT的产热活性来抑制肥胖的发展。
