Okumura Nobuaki, Ikeda Masanori, Satoh Shinya, Dansako Hiromichi, Sugiyama Masaya, Mizokami Masashi, Kato Nobuyuki
Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; Department of Persistent and Oncogenic Viruses, Center for Chronic Viral Disease, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan; Department of Persistent and Oncogenic Viruses, Center for Chronic Viral Disease, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
FEBS Lett. 2015 Apr 28;589(10):1112-8. doi: 10.1016/j.febslet.2015.03.022. Epub 2015 Mar 30.
Hepatitis B virus (HBV) replication is controlled by liver-enriched transcriptional factors, including forkhead box protein A (FOXA) members. Here, we found that FOXA members are directly and indirectly involved in HBV replication in human hepatic cells. HBV replication was elevated in HuH-7 treated with individual FOXA members-specific siRNA. Reciprocally, the downregulation of HBV replication was observed in FOXA-induced HuH-7. However, the mechanism of downregulation is different among FOXA members at the level of HBV RNA transcription, such as precore/pg RNA and 2.1 kb RNA. In addition, FOXA1 and FOXA2 suppressed nuclear hormone receptors, such as HNF4α, that are related to HBV replication.
乙型肝炎病毒（HBV）的复制受肝脏富集转录因子的控制，包括叉头框蛋白A（FOXA）家族成员。在此，我们发现FOXA家族成员直接或间接参与人类肝细胞中的HBV复制。用单个FOXA家族成员特异性siRNA处理的HuH-7细胞中，HBV复制增加。相反，在FOXA诱导的HuH-7细胞中观察到HBV复制下调。然而，在HBV RNA转录水平，如前核心/pg RNA和2.1 kb RNA，FOXA家族成员下调HBV复制的机制有所不同。此外，FOXA1和FOXA2抑制了与HBV复制相关的核激素受体，如肝细胞核因子4α（HNF4α）。
