C57BL/6J小鼠基因组上重复元件阵列的时空重排。
Temporal and spatial rearrangements of a repetitive element array on C57BL/6J mouse genome.
作者信息
Lee Kang-Hoon, Yee Lisa, Lim Debora, Greenhalgh David, Cho Kiho
机构信息
Department of Surgery, University of California, Davis and Shriners Hospitals for Children Northern California, Sacramento, CA 95817, USA.
Department of Surgery, University of California, Davis and Shriners Hospitals for Children Northern California, Sacramento, CA 95817, USA.
出版信息
Exp Mol Pathol. 2015 Jun;98(3):439-45. doi: 10.1016/j.yexmp.2015.03.037. Epub 2015 Mar 31.
Repetitive elements (REs) make up the vast majority of the mammalian genomes. We identified species-specific genomic libraries of RE arrays. The non-random configurations of RE arrays suggest their functions. We tested whether RE arrays undergo age- and tissue/cell-specific rearrangements. An RE array of C57BL/6J mice, containing tandem repeats of a mosaic of transposable REs, was selected to examine rearrangements in different ages and tissues. There were marked changes in the array configuration in the genomes of the skin and brain in all mice of six weeks and older, whereas the heart and liver had alterations at 29weeks. The temporal variations were confirmed by identifying putative rearrangement junctions. Temporal and spatial rearrangements of certain RE arrays may contribute to the acquired characteristics of the genome information system.
重复元件（REs）构成了哺乳动物基因组的绝大部分。我们鉴定了RE阵列的物种特异性基因组文库。RE阵列的非随机构型表明了它们的功能。我们测试了RE阵列是否会发生年龄和组织/细胞特异性重排。选择了C57BL/6J小鼠的一个RE阵列，其包含可转座REs镶嵌体的串联重复序列，以检查不同年龄和组织中的重排情况。六周及以上所有小鼠的皮肤和大脑基因组中的阵列构型有明显变化，而心脏和肝脏在29周时出现改变。通过鉴定假定的重排连接点证实了时间变化。某些RE阵列的时间和空间重排可能有助于基因组信息系统的获得性特征。
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