Department of Pediatrics, Fujita Health University School of Medicine.
Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake.
J Infect Dis. 2015 Oct 1;212(7):1014-21. doi: 10.1093/infdis/jiv160. Epub 2015 Apr 3.
Human herpesvirus 6B (HHV-6B) is the causative agent for exanthem subitum. HHV-6B was associated with mesial temporal sclerosis (MTS), leading to mesial temporal lobe epilepsy (MTLE). In this study, we sought to elucidate the pathogenic role of HHV-6B in patients with MTLE.
Seventy-five intractable MTLE patients, including 52 MTS patients and 23 non-MTS patients, were enrolled in this study. Resected hippocampus, amygdala, and mixed samples of amygdala and uncus samples were examined by real-time polymerase chain reaction (PCR) and reverse-transcriptase PCR to detect viral DNA and messenger RNA (mRNA), respectively. Host gene expressions, including neural markers, were measured using the TaqMan Gene Expression Assay.
Detection of HHV-6 DNA was higher in MTS patients than non-MTS patients (median/interquartile range: 19.1/0-89.2 vs 0.0/0.0-0.0 copies/µg DNA; P = .004). HHV-6B viral DNA was determined in 12/27 HHV-6 DNA-positive samples, and no HHV-6B mRNA were detected in all samples. In MTS patients, expression of monocyte chemotactic protein-1 (P = .029) and glial fibrillary acidic protein (P = .043) were significantly higher in the amygdala samples with HHV-6 DNA than those without viral DNA.
This study suggests that HHV-6B may play an important role in the pathogenesis of MTS via modification of host gene expression.
人类疱疹病毒 6B（HHV-6B）是出疹性疾病的病原体。HHV-6B 与内侧颞叶硬化症（MTS）有关，导致内侧颞叶癫痫（MTLE）。在这项研究中，我们试图阐明 HHV-6B 在 MTLE 患者中的致病作用。
本研究纳入了 75 例耐药性 MTLE 患者，包括 52 例 MTS 患者和 23 例非 MTS 患者。通过实时聚合酶链反应（PCR）和逆转录-PCR 分别检测病毒 DNA 和信使 RNA（mRNA），检测海马体、杏仁核和混合的杏仁核和钩突样本中的 HHV-6B。使用 TaqMan 基因表达检测试剂盒测量宿主基因表达，包括神经标记物。
MTS 患者中 HHV-6 DNA 的检出率高于非 MTS 患者（中位数/四分位距：19.1/0-89.2 与 0.0/0.0-0.0 拷贝/µg DNA；P =.004）。在 27 例 HHV-6 DNA 阳性样本中确定了 12 例 HHV-6B 病毒 DNA，所有样本均未检测到 HHV-6B mRNA。在 MTS 患者中，与无病毒 DNA 的样本相比，HHV-6 DNA 阳性的杏仁核样本中单核细胞趋化蛋白-1（P =.029）和胶质纤维酸性蛋白（P =.043）的表达显著升高。
本研究表明，HHV-6B 可能通过改变宿主基因表达在 MTS 的发病机制中发挥重要作用。
