Paniushkina A E, Tsaplina I A, Grigor'eva N V, Kondrat'eva T F
Mikrobiologiia. 2014 Sep-Oct;83(5):552-64.
An aboriginal community of thermophilic acidophilic chemolithotrophic microorganisms (ACM) was isolated from a sample of pyrite gold-bearing flotation concentrateat 45-47 degrees C and pH 1.8-2.0. Compared to an experimental thermoacidophilic microbial consortium formed in the course of cultivation in parallel bioreactors, it had lower rates of iron leaching and oxidation, while its rate of sulfur oxidation was higher. A new thermophilic acidophilic microbial community was obtained by mutual enrichment with the micioorganisms from thie experimental and aboriginal communities during oxidation of sulfide ore flotation concentrate at 47 degrees C. The dominant bacteria of this new ACM community were Acidithiobacillus caldus strains (the most active sulfur oxidizers) and Sulfobacillus thermotolerans strains (active oxidizers of both iron and sulfur), while iron-oxidizing archaea of the family Ferroplasmaceae and heterotrophic bacteria Alicyclobacillus tolerans were the minor components. The new ACM community showed promise for leaching/oxidation of sulfides from flotation concentrates at high pulp density (S:L = 1:4).
从45-47摄氏度、pH值为1.8-2.0的含硫金矿浮选精矿样品中分离出一个嗜热嗜酸化能自养微生物群落（ACM）。与在平行生物反应器中培养过程中形成的实验性嗜热嗜酸微生物联合体相比，其铁浸出和氧化速率较低，而硫氧化速率较高。通过在47摄氏度下对硫化矿浮选精矿进行氧化时，将来自实验群落和原始群落的微生物相互富集，获得了一个新的嗜热嗜酸微生物群落。这个新的ACM群落的优势细菌是嗜酸氧化亚铁硫杆菌菌株（最活跃的硫氧化剂）和嗜热嗜酸硫化叶菌菌株（铁和硫的活跃氧化剂），而铁氧化古菌铁原体科和兼性异养细菌嗜酸耐热脂环酸芽孢杆菌是次要成分。新的ACM群落在高矿浆密度（固液比=1:4）下对浮选精矿中的硫化物进行浸出/氧化方面显示出前景。
