Prasolova M A, Ivanov M K, Gashnikova N M, Netesova E S, Dymshyts G M
Mol Gen Mikrobiol Virusol. 2014(4):20-5.
The RT-PCR method with real-time fluorescence detection was used for development of φ prototype of diagnostic kit for reliable diagnosis of genetic variants of RNA of the HIV-1 of groups M, N, O, P and RNA of the HIV-2 in blood plasma and serum. The kit is stable against nucleotide defects, provides broad linear range of concentration of the HIV RNA, 100% analytical specificity and adequate analytical sensitivity: 42 ME/ml (HIV-1 of group M), 45 copies/ml (HIV-2), 92 copies/ml (HIV-1 of group O), 90 copies/ml (HIV-1 of group N). The kit provided successful detection and measurement of HIV RNA concentration in the samples of the international reference panel of the HIV-1 genotype. The results of the test correlate with results of commercial tests.
采用实时荧光检测的逆转录聚合酶链反应（RT-PCR）方法开发了一种诊断试剂盒的φ原型，用于可靠诊断血浆和血清中M、N、O、P组HIV-1的RNA基因变体以及HIV-2的RNA。该试剂盒对核苷酸缺陷具有稳定性，提供了较宽的HIV RNA浓度线性范围、100%的分析特异性和足够的分析灵敏度：42拷贝/ml（M组HIV-1）、45拷贝/ml（HIV-2）、92拷贝/ml（O组HIV-1）、90拷贝/ml（N组HIV-1）。该试剂盒成功检测并测量了HIV-1基因型国际参考品样本中的HIV RNA浓度。检测结果与商业检测结果相关。
