Polymer Electronics Research Centre, School of Chemical Sciences, University of Auckland, 23 Symonds Street, Auckland, New Zealand.
School of Chemical and Physical Sciences, Victoria University of Wellington, Wellington 6012, New Zealand; The MacDiarmid Institute for Advanced Materials and Nanotechnology, New Zealand.
Biosens Bioelectron. 2015 Aug 15;70:398-403. doi: 10.1016/j.bios.2015.03.050. Epub 2015 Mar 24.
We report an electrochemical aptasensor for the rapid, label-free detection of 17β-estradiol (E2) from femtomolar to micromolar levels. The sensor features an aptamer-functionalised nanoporous conducting polymer electrode whose surface potential is probed via electrochemical impedance spectroscopy. The unprecedented detection limit for E2 is explained via the redistribution of negative charges in the electrode double-layer region when the aptamer adopts a folded conformation around the small neutral target molecule. The sensor responds approximately logarithmically over a wide dynamic range of E2 concentration that spans biological trigger levels, with excellent discrimination against structurally similar molecules including progesterone, and robust operation in human urine. The generality of the approach of using conformationally gated small molecule binding aptamers is highlighted with a further example of adenosine detection via the adenosine binding aptamer.
我们报道了一种电化学适体传感器，用于快速、无标记地检测从飞摩尔到微摩尔级别的 17β-雌二醇（E2）。该传感器采用适体功能化的纳米多孔导电聚合物电极，通过电化学阻抗谱探测其表面电势。E2 的空前检测极限可以通过当适体围绕小分子中性靶分子折叠时，在电极双电层区域内负电荷的重新分布来解释。该传感器在跨越生物触发水平的宽 E2 浓度动态范围内呈近似对数响应，对包括孕酮在内的结构相似分子具有出色的区分能力，并且在人尿中具有稳健的操作性能。通过使用构象门控小分子结合适体的方法的通用性，通过腺苷结合适体检测腺苷的进一步示例得到了强调。
