Sonomura Kazuhiro, Kudoh Shinobu, Sato Taka-Aki, Matsuda Fumihiko
Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan.
J Sep Sci. 2015 Jun;38(12):2033-7. doi: 10.1002/jssc.201401440. Epub 2015 May 8.
A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono-hexsosyl ceramide groups with good peak area repeatability (RSD% < 10) and linearity (R(2) > 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co-existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage.
采用亲水作用液相色谱-电喷雾电离串联质谱法开发了一种分析内源性脂质及相关化合物的新方法。具有氨甲酰固定相的亲水作用液相色谱实现了磷脂酰胆碱、溶血磷脂酰胆碱、鞘磷脂、神经酰胺和单己糖神经酰胺基团的清晰分离，具有良好的峰面积重复性（相对标准偏差%<10）和线性（R²>0.99）。所建立的方法应用于人体血浆分析，成功检测并可重复鉴定了总共117种内源性脂质。此外，我们研究了在与脂质的单次分析运行中处理的相同生物样品中共存的小极性代谢物（如氨基酸和有机酸）的同时检测。我们的结果表明，亲水作用液相色谱是人体血浆脂质组分析的有用工具，并且提供了更全面的代谢组覆盖范围。
