Oke Yoshihiko, Boiroux Dimitri, Miwakeichi Fumikazu, Oku Yoshitaka
Division of Physiome, Department of Physiology, Hyogo College of Medicine, Mukogawacho 1-1, Nishinomiya, Hyogo 663-8501, Japan.
Division of Physiome, Department of Physiology, Hyogo College of Medicine, Mukogawacho 1-1, Nishinomiya, Hyogo 663-8501, Japan.
Neurosci Lett. 2015 May 19;595:12-7. doi: 10.1016/j.neulet.2015.04.003. Epub 2015 Apr 4.
In the pre-Bötzinger complex of the ventral medulla (preBötC), a variable pattern of inspiratory neuronal output and synchronous activation of inspiratory cells can be observed. However, it is not well known whether cellular activation patterns among inspiratory cells are variable or fixed. Here, we evaluated the activation sequence of inspiratory cells during individual rhythmic bursts using calcium imaging. Onset timing and peak timing of calcium fluctuations during rhythmic bursts in individual inspiratory cells were used to evaluate the activation sequence. The sequences of both timings changed stochastically in individual rhythmic bursts, although the sequences differed between the two timings even within the same rhythmic burst. The weak correlation between these two timings might indicate that the two parameters reflect different physiological events. Furthermore, a subset of inspiratory cells was found to initially activate in the sequences of successive rhythmic bursts. These results suggest that rhythmic activation of inspiratory cells occurs with a degree of loose regularity but is not invariable with respect to the sequence of either onset or peak timing.
在延髓腹侧的前包钦格复合体（preBötC）中，可以观察到吸气神经元输出的可变模式以及吸气细胞的同步激活。然而，吸气细胞之间的细胞激活模式是可变的还是固定的，目前尚不清楚。在这里，我们使用钙成像评估了单个节律性爆发期间吸气细胞的激活顺序。单个吸气细胞节律性爆发期间钙波动的起始时间和峰值时间用于评估激活顺序。尽管在同一节律性爆发中这两个时间的顺序不同，但在单个节律性爆发中，这两个时间的顺序都随机变化。这两个时间之间的弱相关性可能表明这两个参数反映了不同的生理事件。此外，发现一部分吸气细胞在连续节律性爆发的序列中最初被激活。这些结果表明，吸气细胞的节律性激活在一定程度上具有松散的规律性，但在起始或峰值时间的序列方面并非一成不变。
