Nir H, Berkovitch M, Youngster I, Kozer E, Goldman M, Abu-Kishk I
Pediatric Division, Assaf Harofeh Medical Center Zerifin 70300, Sackler Faculty of Medicine Tel Aviv University, Israel -
Minerva Urol Nefrol. 2015 Jun;67(2):97-102.
Acute renal injury may occur after amphotericin B (AmB) administration. The hypothesized injury mechanism is renal vasoconstriction and direct toxic damage. Hyperbaric oxygen therapy (HBO) is indicated for treatment of many ischemic events but not for acute renal failure (ARF). The aim of this study was to investigate the role of HBO therapy in AmB induced ARF.
ARF was induced in 41 Sprague-Dawley rats by a single dose of 75 mg/kg AmB. The rats were randomly divided into two groups; one group was treated with daily HBO for 3 consecutive days. The control group received no HBO treatment. Parameters of renal function were taken on the 5th day after AmB administration.
Forty-one rats were treated with AmB, 21 received HBO and 20 served as controls. Body weight loss following the administration of AmB was 13.5+14.7% in the HBO treated rats, as opposed to 24.6+5% in the control group (P=0.004). Serum creatinine and urea were 0.49+0.13 mg/dL and 200.63+87.82 mg/dL in the treatment group and 0.70+0.22 mg/dL and 368.01+169.35 mg/dL, respectively in the control (P=0.001).
In this model of AmB-induced ARF, HBO treatment alleviated renal injury as reflected by changes in serum creatinine and urea levels.
两性霉素B（AmB）给药后可能发生急性肾损伤。推测的损伤机制是肾血管收缩和直接毒性损伤。高压氧疗法（HBO）适用于治疗许多缺血性事件，但不适用于急性肾衰竭（ARF）。本研究的目的是探讨HBO疗法在AmB诱导的ARF中的作用。
41只Sprague-Dawley大鼠单次给予75mg/kg AmB诱导ARF。大鼠被随机分为两组；一组连续3天每日接受HBO治疗。对照组未接受HBO治疗。在给予AmB后第5天测定肾功能参数。
41只大鼠接受了AmB治疗，21只接受了HBO治疗，20只作为对照。接受HBO治疗的大鼠在给予AmB后体重减轻13.5+14.7%，而对照组为24.6+5%（P=0.004）。治疗组血清肌酐和尿素分别为0.49+0.13mg/dL和200.63+87.82mg/dL，对照组分别为0.70+0.22mg/dL和368.01+169.35mg/dL（P=0.001）。
在这个AmB诱导的ARF模型中，HBO治疗减轻了肾损伤，血清肌酐和尿素水平的变化反映了这一点。
