Nakajima H
Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kanagawa, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1989 Oct;63(10):1221-7.
To clarify the relationship between oncogene c-sis expression and tumorigenesis in human osteosarcoma, in situ hybridization and immunohistochemical studies were performed to detect c-sis mRNA and PDGF-like protein partially consisting of c-sis product. Formalin fixed-paraffin embedded sections of eight cases of osteosarcoma of bone were examined. Three osteosarcomas highly expressed c-sis mRNA with a fine granular pattern in their cytoplasms. Five osteosarcomas, including three cases with c-sis expression, contained PDGF-like protein in their cytoplasms. These results suggested that c-sis oncogene and PDGF-like protein were closely related to tumorigenesis in human osteosarcoma. The DNA-mRNA in situ hybridization technique applied by the author is as efficient as the immunohistochemical method in cancer research.
为阐明癌基因c-sis表达与人类骨肉瘤发生之间的关系，进行了原位杂交和免疫组化研究，以检测c-sis mRNA和部分由c-sis产物组成的血小板衍生生长因子（PDGF）样蛋白。对8例骨骨肉瘤的福尔马林固定石蜡包埋切片进行了检查。3例骨肉瘤在其细胞质中高表达c-sis mRNA，呈细颗粒状。5例骨肉瘤，包括3例有c-sis表达的病例，其细胞质中含有PDGF样蛋白。这些结果表明，c-sis癌基因和PDGF样蛋白与人类骨肉瘤的发生密切相关。作者应用的DNA-mRNA原位杂交技术在癌症研究中与免疫组化方法一样有效。
