Kanai M, Watari N, Hashitani T, Shiraya N
J Electron Microsc (Tokyo). 1989;38(5):371-81.
In a male patient suffering from congenital ectodermal dysplasia with sweating only on the plantar and palmar surface, we found that the anhidrotic eccrine sweat glands became able to produce sweat after repeated local application of acetylcholine. We ultrastructurally examined the sweat glands in skin biopsies before and after acetylcholine treatment. The secretory portion of the sweat glands of the right plantar region was characterized by well-developed basal infoldings and intercellular interdigitations, and also by dilated intercellular spaces and canaliculi. In the flexor region of the right brachium (anhidrotic area), the secretory portion showed hypoplastic features, especially denoted by the presence of few locally distributed basal infoldings and intercellular canaliculi. The secretory cells seemed to be hypofunctional with the nuclei containing much heterochromatin. After treatment, in the flexor region of the left brachium and the right infraclavicular region, where sweating was induced, the secretory portion appeared to be considerably activated, though hypoplastic structures were unchanged: the secretory cell nuclei contained dispersed chromatin, the Golgi apparatus in the dark cells was well developed, and both in the clear cells and the myoepithelial cells micropinocytotic vesicle-like structures increased in number. In ductular cells, mitochondria also increased in number.
在一名患有先天性外胚层发育不良且仅足底和手掌表面出汗的男性患者中，我们发现，在反复局部应用乙酰胆碱后，无汗的小汗腺能够产生汗液。我们对乙酰胆碱治疗前后皮肤活检中的汗腺进行了超微结构检查。右足底区域汗腺的分泌部具有发达的基底褶和细胞间指状突，细胞间间隙和小管也扩张。在右肱部屈侧区域（无汗区），分泌部表现出发育不全的特征，尤其是局部分布的基底褶和细胞间小管较少。分泌细胞似乎功能低下，细胞核含有大量异染色质。治疗后，在诱导出汗的左肱部屈侧区域和右锁骨下区域，尽管发育不全的结构未改变，但分泌部似乎被显著激活：分泌细胞核含有分散的染色质，暗细胞中的高尔基体发育良好，透明细胞和肌上皮细胞中的微吞饮小泡样结构数量均增加。在导管细胞中，线粒体数量也增加。
