Lyons Rebecca, Stiller Jiri, Powell Jonathan, Rusu Anca, Manners John M, Kazan Kemal
CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.
CSIRO Agriculture Flagship, Black Mountain Laboratories, Canberra, ACT, Australia.
PLoS One. 2015 Apr 7;10(4):e0121902. doi: 10.1371/journal.pone.0121902. eCollection 2015.
Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.
一些最具毁灭性的农业病害是由感染根部的病原体引起的，但迄今为止，关于这些相互作用的大多数研究都集中在地上组织的宿主反应上，而不是地下组织。尖孢镰刀菌是一种感染根部的病原体，可在包括拟南芥在内的多种植物物种上引起枯萎病。为了研究和比较尖孢镰刀菌在不同拟南芥组织中引发的转录变化，我们用尖孢镰刀菌感染土壤种植的植物，并对在早期和晚期时间点收获的根和叶组织进行RNA测序分析。至少一半由尖孢镰刀菌诱导或抑制的基因表现出组织特异性调控。生长素和脱落酸信号转导的调节因子、甘露糖结合凝集素和过氧化物酶在根组织中表现出强烈的差异表达。我们证明，在根中受调控的两个基因ARF2和PRX33促进了对尖孢镰刀菌的易感性。在叶片中，防御素以及与生长素反应、寒冷和衰老相关的基因受到强烈调控，而茉莉酸生物合成和信号转导基因在整个植物中被诱导。
