Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India.
Int J Biol Macromol. 2015;78:62-71. doi: 10.1016/j.ijbiomac.2015.03.050. Epub 2015 Apr 4.
A multi technique approach was utilized to explore the interaction between a novel green gemini surfactant, ethane-1,2-diyl bis(N,N-dimethyl-N-tetradecylammoniumacetoxy) dichloride (14-E2-14), with bovine milk xanthine oxidase (XO). Tensiometric, spectroscopic, microscopic and molecular modeling results demonstrate significant interaction and structural change of native xanthine oxidase upon 14-E2-14 combination. The results obtained in this study may be beneficial for scientists to calibrate conformation of the enzyme by novel biodegradable/green microstructures; consequently, it would likely add new impetus in understanding the treatment modes of various diseases like gout, hyperuricemia, liver and brain necrosis. Moreover, the 14-E2-14-XO interaction assists to unfurl new routes in the designing/selection of green-surfactant-protein mixtures widely used in food processing, cosmetics, and pharmaceutical industries.
采用多种技术手段研究了新型绿色Gemini 表面活性剂乙烷-1,2-双（N,N-二甲基-N-十四烷基铵乙氧基）二氯化物（14-E2-14）与牛乳黄嘌呤氧化酶（XO）之间的相互作用。张力、光谱、显微镜和分子建模结果表明，14-E2-14 结合后，天然黄嘌呤氧化酶发生了显著的相互作用和结构变化。本研究的结果可能有助于科学家通过新型可生物降解/绿色微观结构来校准酶的构象；因此，它可能会为理解治疗各种疾病（如痛风、高尿酸血症、肝坏死和脑坏死）的模式提供新的动力。此外，14-E2-14-XO 的相互作用有助于在食品加工、化妆品和制药行业广泛使用的绿色表面活性剂-蛋白质混合物的设计/选择方面开辟新途径。
