Fan Xing-hua, Shi Wei-Zhou, Cheng Yun-xiang, Zou Kai-jie, Yang Xiu-fen
Zhongguo Zhong Yao Za Zhi. 2014 Nov;39(22):4453-8.
Xuefu Zhuyu decoction (XFZYD) is a famous traditional Chinese medicine (TCM) formula, is widely used in the treatment of cardiovascular and cerebrovascular diseases in China over one hundred years. But its effect on antioxidant and drug-metabolizing enzymes are unknown. This study was to observe the effects of Xuefu Zhuyu decoction (XFZYD) on the activities of antioxidant and drug metabolism enzymes (DMEs) in liver of rats. Male SD rats, treated with XFZYD at the dosage of 3.51, 7.02 and 14.04 g x kg(-1) per day for 15 days, serum were collected, tissue fluid, cytosols and microsomes isolated from liver tissues were prepared by centrifugation according to the standard procedure, the activities of antioxidant enzymes and drug-Metabolizing Enzymes were determined by UV-V is spectrophotometer. In serum, the activities of AST was not significantly affected by the treatment with XFZYD, at the high- est dose, the levels of ALT, Cr and BUN were significantly decreased (P < 0.05). GPX were significantly increased at the dose of 7.02, 14.04 g x kg(-1) (P < 0.05), CAT were significantly increased at the highest dose (P < 0.05). T-SOD was not significantly af- fected by this treatment. In the liver tissue, GPX was significantly increased at the dose of 3.51, 7.02 g x kg(-1) (P < 0.05), GST, CAT and T-SOD were not significantly affected following this treatment. In cytosols, GST was significantly increased at the dose of 3.51 g x kg(-1) (P < 0.05), T-SOD was remarkable induced at the dose of 3.51 and 7.02 g x kg(-1) (P < 0.05). In microsomes, XFZYD had no significant effect on Cytochromeb5, NADPH-Cytochrome P450 reductase, CYP3A, CYP2E1 and UGT, XFZYD significantly in- duced GST at the dose of 3.51 and 7.02 g x kg(-1) (P < 0.05), and the level of GSH were significantly increased by XFZYD at the dose of 3.51, 7.02 and 14.04 g kg(-1) (P < 0.05). These findings suggest XFZYD can induce the activities of GPX, CAT, SOD, GST and increase GSH level in liver of rats, which indicate XFZYD may have detoxification and antioxidant functions.
血府逐瘀汤（XFZYD）是一种著名的中药方剂，在我国治疗心脑血管疾病已有一百多年的广泛应用历史。但其对抗氧化酶和药物代谢酶的作用尚不清楚。本研究旨在观察血府逐瘀汤（XFZYD）对大鼠肝脏抗氧化酶和药物代谢酶（DMEs）活性的影响。雄性SD大鼠，每天按3.51、7.02和14.04 g·kg⁻¹的剂量给予血府逐瘀汤（XFZYD）处理15天，收集血清，按照标准程序通过离心从肝组织中分离制备组织液、胞质溶胶和微粒体，采用紫外 - 可见分光光度计测定抗氧化酶和药物代谢酶的活性。在血清中，血府逐瘀汤（XFZYD）处理对AST活性无显著影响，在最高剂量时，ALT、Cr和BUN水平显著降低（P < 0.05）。在7.02、14.04 g·kg⁻¹剂量时GPX显著升高（P < 0.05），在最高剂量时CAT显著升高（P < 0.05）。T - SOD不受该处理的显著影响。在肝组织中，在3.51、7.02 g·kg⁻¹剂量时GPX显著升高（P < 0.05），该处理后GST、CAT和T - SOD未受显著影响。在胞质溶胶中，在3.51 g·kg⁻¹剂量时GST显著升高（P < 0.05），在3.51和7.02 g·kg⁻¹剂量时T - SOD显著诱导（P < 0.05）。在微粒体中，血府逐瘀汤（XFZYD）对细胞色素b5、NADPH - 细胞色素P450还原酶、CYP3A、CYP2E1和UGT无显著影响，在3.51和7.02 g·kg⁻¹剂量时血府逐瘀汤（XFZYD）显著诱导GST（P < 0.05），在3.51、7.02和14.04 g·kg⁻¹剂量时血府逐瘀汤（XFZYD）显著提高GSH水平（P < 0.05）。这些发现表明血府逐瘀汤（XFZYD）可诱导大鼠肝脏中GPX、CAT、SOD、GST的活性并提高GSH水平，这表明血府逐瘀汤（XFZYD）可能具有解毒和抗氧化功能。
