激活素A可诱导人孤雌生殖胚胎干细胞分化为定形内胚层。
Activin A can induce definitive endoderm differentiation from human parthenogenetic embryonic stem cells.
作者信息
Wang Zhiqiang, Li Wenliang, Chen Tianxing, Yang Jun, Wen Zhengqi, Yan Xinmin, Shen Tao, Liang Rui
机构信息
Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People's Republic of China.
出版信息
Biotechnol Lett. 2015 Aug;37(8):1711-7. doi: 10.1007/s10529-015-1829-x. Epub 2015 Apr 8.
OBJECTIVES
As activin/nodal signaling plays a key role in definitive endoderm (DE) differentiation, we have explored activin A-induced differentiation of DE from human parthenogenetic embryonic stem cells (hPESCs).
RESULTS
Administration of 5 ng activin A/ml had no effect on the expression of markers of DE differentiation. However, higher concentrations of activin A (50 and 100 ng/ml) upregulated Sox17 and Cxcr4, as well upregulating the mesendodermal precursor marker, Brachyury.
CONCLUSIONS
These findings demonstrate that low dose activin A can maintain the undifferentiated potency of hPESCs, whereas higher doses induce DE differentiation; 50 ng/ml is the optimal concentration for inducing DE from hPESCs.
目的
由于激活素/节点信号通路在确定内胚层（DE）分化中起关键作用，我们探索了激活素A诱导人孤雌生殖胚胎干细胞（hPESCs）向DE分化的情况。
结果
每毫升添加5纳克激活素A对DE分化标志物的表达没有影响。然而，更高浓度的激活素A（50和100纳克/毫升）上调了Sox17和Cxcr4，同时也上调了中胚层内胚层前体标志物Brachyury。
结论
这些发现表明，低剂量激活素A可维持hPESCs的未分化潜能，而高剂量则诱导DE分化；50纳克/毫升是诱导hPESCs向DE分化的最佳浓度。
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