Giribabu Nelli, Eswar Kumar Kilari, Swapna Rekha Somesula, Muniandy Sekaran, Salleh Naguib
Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Pharmacology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh 530 003, India.
Evid Based Complement Alternat Med. 2015;2015:542026. doi: 10.1155/2015/542026. Epub 2015 Mar 17.
The effect of V. vinifera seeds on carbohydrate metabolizing enzymes and other enzymes of the liver in diabetes is currently unknown. We therefore investigated changes in the activity levels of these enzymes following V. vinifera seed extract administration to diabetic rats. Methods. V. vinifera seed ethanolic extract (250 and 500 mg/kg/day) or glibenclamide (600 μg/kg/day) was administered to streptozotocin-induced male diabetic rats for 28 consecutive days. At the end of treatment, liver was harvested and activity levels of various liver enzymes were determined. Levels of thiobarbituric acid reactive substances (TBARS) were measured in liver homogenates and liver histopathological changes were observed. Results. V. vinifera seed ethanolic extract was able to prevent the decrease in ICDH, SDH, MDH, and G-6-PDH and the increase in LDH activity levels in liver homogenates. The seed extract also caused serum levels of ALT, AST, ALP, ACP, GGT, and total bilirubin to decrease while causing total proteins to increase. Additionally, the levels of ALT, AST, and TBARS in liver homogenates were decreased. Histopathological changes in the liver were reduced. Conclusion. Near normal activity levels of various enzymes and histology of the liver following V. vinifera seed ethanolic extract administration may be due to decrease in liver oxidative stress in diabetes.
目前尚不清楚酿酒葡萄种子对糖尿病患者肝脏中碳水化合物代谢酶及其他酶的影响。因此，我们研究了给糖尿病大鼠灌胃酿酒葡萄种子提取物后这些酶活性水平的变化。方法：将酿酒葡萄种子乙醇提取物（250和500毫克/千克/天）或格列本脲（600微克/千克/天）连续28天给予链脲佐菌素诱导的雄性糖尿病大鼠。治疗结束时，采集肝脏并测定各种肝酶的活性水平。测定肝匀浆中硫代巴比妥酸反应性物质（TBARS）的水平，并观察肝脏组织病理学变化。结果：酿酒葡萄种子乙醇提取物能够防止肝匀浆中异柠檬酸脱氢酶（ICDH）、琥珀酸脱氢酶（SDH）、苹果酸脱氢酶（MDH）和葡萄糖-6-磷酸脱氢酶（G-6-PDH）活性降低以及乳酸脱氢酶（LDH）活性水平升高。种子提取物还使血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、酸性磷酸酶（ACP）、γ-谷氨酰转肽酶（GGT）和总胆红素水平降低，同时使总蛋白水平升高。此外，肝匀浆中ALT、AST和TBARS的水平降低。肝脏的组织病理学变化减轻。结论：给予酿酒葡萄种子乙醇提取物后，各种酶的活性水平接近正常以及肝脏组织学正常，可能是由于糖尿病时肝脏氧化应激降低所致。
