Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Environ Health Prev Med. 2021 Mar 22;26(1):38. doi: 10.1186/s12199-021-00962-0.
Periploca aphylla is used by local population and indigenous medicine practitioners as stomachic, tonic, antitumor, antiulcer, and for treatment of inflammatory disorders. The aim of this study was to evaluate antidiabetic effect of the extract of P. aphylla and to investigate antioxidant and hypolipidemic activity in streptozotocin (STZ)-induced diabetic rats.
The present research was conducted to evaluate the antihyperglycemic potential of methanol extract of P. aphylla (PAM) and subfractions n-hexane (PAH), chloroform (PAC), ethyl acetate (PAE), n-butanol (PAB), and aqueous (PAA) in glucose-overloaded hyperglycemic Sprague-Dawley rats. Based on the efficacy, PAB (200 mg/kg and 400 mg/kg) was tested for its antidiabetic activity in STZ-induced diabetic rats. Diabetes was induced via intraperitoneal injection of STZ (55 mg/kg) in rat. Blood glucose values were taken weekly. HPLC-DAD analysis of PAB was carried out for the presence of various polyphenols.
HPLC-DAD analysis of PAB recorded the presence of rutin, catechin, caffeic acid, and myricetin. Oral administration of PAB at doses of 200 and 400 mg/kg for 21 days significantly restored (P < 0.01) body weight (%) and relative liver and relative kidney weight of diabetic rats. Diabetic control rats showed significant elevation (P < 0.01) of AST, ALT, ALP, LDH, total cholesterol, triglycerides, LDL, creatinine, total bilirubin, and BUN while reduced (P < 0.01) level of glucose, total protein, albumin, insulin, and HDL in serum. Count of blood cells and hematological parameters were altered in diabetic rats. Further, glutathione peroxidase, catalase, superoxide dismutase, glutathione reductase, and total soluble protein concentration decreased while concentration of thiobarbituric acid reactive substances and percent DNA damages increased (P < 0.01) in liver and renal tissues of diabetic rats. Histopathological damage scores increased in liver and kidney tissues of diabetic rats. Intake of PAB (400 mg/kg) resulted in significant improvement (P < 0.01) of above parameters, and results were comparable to that of standard drug glibenclamide.
The result suggests the antihyperglycemic, antioxidant, and anti-inflammatory activities of PAB treatment in STZ-compelled diabetic rat. PAB might be used as new therapeutic agent in diabetic patients to manage diabetes and decrease the complications.
白首乌被当地居民和传统医学从业者用作胃药、滋补剂、抗肿瘤、抗溃疡以及治疗炎症性疾病。本研究旨在评估白首乌提取物的降血糖作用，并研究其在链脲佐菌素（STZ）诱导的糖尿病大鼠中的抗氧化和降血脂活性。
本研究旨在评估甲醇提取物白首乌（PAM）及其正己烷（PAH）、氯仿（PAC）、乙酸乙酯（PAE）、正丁醇（PAB）和水（PAA）部分对葡萄糖超负荷高血糖 Sprague-Dawley 大鼠的降血糖潜力。基于疗效，用白首乌正丁醇提取物（PAB）（200mg/kg 和 400mg/kg）在 STZ 诱导的糖尿病大鼠中测试其降血糖活性。通过腹腔注射 STZ（55mg/kg）诱导大鼠糖尿病。每周测量血糖值。对 PAB 进行 HPLC-DAD 分析，以确定各种多酚的存在。
PAB 的 HPLC-DAD 分析记录了芦丁、儿茶素、咖啡酸和杨梅素的存在。PAB 口服给药 21 天，剂量为 200mg/kg 和 400mg/kg，可显著恢复（P<0.01）糖尿病大鼠的体重（%）和相对肝重和相对肾重。糖尿病对照组大鼠血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、总胆固醇、甘油三酯、低密度脂蛋白、肌酐、总胆红素和尿素氮显著升高（P<0.01），而血清中葡萄糖、总蛋白、白蛋白、胰岛素和高密度脂蛋白水平显著降低（P<0.01）。糖尿病大鼠的血细胞计数和血液学参数发生改变。此外，肝和肾组织中谷胱甘肽过氧化物酶、过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶和总可溶性蛋白浓度降低，而肝和肾组织中丙二醛反应性物质浓度和 DNA 损伤百分比增加（P<0.01）。糖尿病大鼠肝、肾组织的组织病理学损伤评分增加。PAB（400mg/kg）的摄入可显著改善（P<0.01）上述参数，结果与标准药物格列本脲相当。
结果表明，PAB 治疗 STZ 诱导的糖尿病大鼠具有降血糖、抗氧化和抗炎活性。PAB 可作为治疗糖尿病患者的新型治疗剂，以控制糖尿病并减少并发症。
