Liu Shengjie, Chen Deyu, Zhang Qiuyan, Ye Hong, Zhu Maoying
School of Biology and Food Engineering, Department of Food, College of Information Engineering, Fuyang Normal College, Fuyang 236037, China.
School of Biology and Food Engineering, Fuyang Normal College, Fuyang 236037, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Apr;31(4):462-6.
To explore the cross immunogenicity between Ia-associated invariant chain (Ii) of chicken (CIi) and Ii of Muscovy duck (MDIi).
The immunoreactivity between mouse anti-CIi serum and the MDIi expressed by prokaryotic cell, and the immunoreactivity between the mouse anti-MDIi serum and the CIi expressed by prokaryotic cell were detected through indirect ELISA. To detect the immunoreactivity between the mouse anti-CIi serum and the MDIi of the spleen tissues of Muscovy duck, and the immunoreactivity between the mouse anti-MDIi serum and the CIi of the liver tissues of chicken (tissular CIi), the fluorescence immunohistochemistry assay was performed using DyLight 488 affiniPure goat anti-mouse IgG as the second antibody. The fluorescence intensity was detected in each tissue by Leica fluorescence microscopic imaging system.
The titer of ELISA reactivity between mouse anti-MDIi serum and the expressed CIi was 1:8000. The titer of ELISA reactivity between mouse anti-CIi serum and the expressed MDIi was 1:16 000. The mouse anti-CIi serum and anti-MDIi serum could respectively react with tissular MDIi and tissular CIi, showing visible green fluorescence in corresponding cells of each tissue. But the fluorescence intensities in the above tissues were weaker than those in positive control tissues in which the mouse anti-MDIi serum or anti-CIi serum reacted with tissular MDIi or tissular CIi respectively.
The cross immunogenicity between CIi and MDIi has been verified through the immunological experiments. The molecular basis of common antigen epitope formation between CIi and MDIi involves the conservation of the two Ii sequences, which reveals the high conservative property of Ii among variant birds from the perspective of immunology.
探讨鸡Ia相关恒定链（CIi）与番鸭Ii（MDIi）之间的交叉免疫原性。
通过间接ELISA检测小鼠抗CIi血清与原核细胞表达的MDIi之间的免疫反应性，以及小鼠抗MDIi血清与原核细胞表达的CIi之间的免疫反应性。为检测小鼠抗CIi血清与番鸭脾脏组织中MDIi之间的免疫反应性，以及小鼠抗MDIi血清与鸡肝脏组织中CIi（组织CIi）之间的免疫反应性，以DyLight 488亲和纯山羊抗小鼠IgG作为二抗进行荧光免疫组织化学检测。使用徕卡荧光显微镜成像系统检测各组织中的荧光强度。
小鼠抗MDIi血清与表达的CIi之间ELISA反应的效价为1:8000。小鼠抗CIi血清与表达的MDIi之间ELISA反应的效价为1:16000。小鼠抗CIi血清和抗MDIi血清可分别与组织MDIi和组织CIi反应，在各组织的相应细胞中呈现可见的绿色荧光。但上述组织中的荧光强度低于阳性对照组织，在阳性对照组织中，小鼠抗MDIi血清或抗CIi血清分别与组织MDIi或组织CIi反应。
通过免疫学实验验证了CIi与MDIi之间的交叉免疫原性。CIi与MDIi之间共同抗原表位形成的分子基础涉及两个Ii序列的保守性，这从免疫学角度揭示了Ii在不同禽类中的高度保守性。
