Hirai K, Shimomura T, Ishizaka S, Shimokawa M, Kitaguchi K, Yamagishi N, Kuro M, Okuda T
Masui. 1989 Oct;38(10):1335-40.
Sequential in vitro lymphocyte function tests in 13 patients undergoing cardiac operation were performed to study B lymphocyte function following operation. Lymphocytes were stimulated with phytohemagglutinin (PHA), pokeweed mitogen (PWM) and Staphylococcus aureus Cowan 1 (SAC). Mitogen responses were measured by 3H-labeled thymidine incorporation. The SAC responses were significantly depressed following operation. Immunoglobulin secreting cells were measured by protein A plaque forming cell assay. The numbers of immunoglobulin secreting cells induced by PWM or SAC decreased remarkably at least as long as 3 day after the operation. The percentage of circulating B lymphocytes increased significantly postoperatively. This indicates that the B lymphocytes remaining after the operation were functionally impaired.
对13例接受心脏手术的患者进行了连续的体外淋巴细胞功能测试，以研究术后B淋巴细胞功能。用植物血凝素（PHA）、商陆有丝分裂原（PWM）和金黄色葡萄球菌Cowan 1（SAC）刺激淋巴细胞。通过3H标记的胸腺嘧啶核苷掺入法测量有丝分裂原反应。术后SAC反应明显降低。通过蛋白A斑形成细胞试验测量免疫球蛋白分泌细胞。术后至少3天内，由PWM或SAC诱导的免疫球蛋白分泌细胞数量显著减少。术后循环B淋巴细胞百分比显著增加。这表明术后剩余的B淋巴细胞功能受损。
