Yao Feng, Yi Bin, Shen Caihong, Tao Fei, Liu Yumin, Lin Zhixin, Xu Ping
State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
National Engineering Research Center of Solid-State Brewing, Luzhou 646000, People's Republic of China.
Sci Rep. 2015 Apr 10;5:9553. doi: 10.1038/srep09553.
Luzhoulaojiao liquor is a type of Chinese liquor that dates back hundreds of years, but whose precise chemical composition remains unknown. This paper describes the screening of the liquor and the identification of its compounds using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC × GC/TOF-MS). Samples were prepared by both liquid-liquid extraction and solid-phase microextraction, which facilitated the detection of thousands of compounds in the liquor, thus demonstrating the superior performance of the proposed method over those reported in previous studies. A total of 320 compounds were common to all 18 types of Luzhoulaojiao liquor studied here, and 13 abundant and potentially bioactive compounds were further quantified. The results indicated that the high-performance method presented here is well suited for the detection and identification of compounds in liquors. This study also contributes to enriching our knowledge of the contents of Chinese liquors.
泸州老窖酒是一种可追溯至数百年前的中国白酒，但其确切的化学成分仍不为人知。本文描述了使用全二维气相色谱/飞行时间质谱法（GC×GC/TOF-MS）对该酒进行筛选及对其化合物进行鉴定的过程。通过液液萃取和固相微萃取两种方法制备样品，这有助于检测出该酒中的数千种化合物，从而证明了所提出的方法比先前研究中报道的方法具有更优越的性能。在本文研究的所有18种泸州老窖酒中，共有320种化合物是共有的，并且对13种含量丰富且具有潜在生物活性的化合物进行了进一步定量分析。结果表明，本文提出的高性能方法非常适合用于酒类化合物的检测和鉴定。这项研究也有助于丰富我们对中国白酒成分的认识。
