Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 675-8501, Japan.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
Bioresour Technol. 2015 Jun;186:351-355. doi: 10.1016/j.biortech.2015.03.127. Epub 2015 Mar 31.
Sequential batch fermentation from sweet sorghum juice concentrated by membrane separation (ultrafiltration permeation and nanofiltration concentration) to increase sugar contents, was investigated. Ethanol production at 5th batch fermentation by Saccharomyces cerevisiae BY4741 attained 113.7±3.1 g L(-1) (89.1±2.2% of the theoretical ethanol yield) from 270.0±22.6 g L(-1) sugars, corresponding to 98.7% of ethanol titer attained at the 1st batch fermentation. This titer was comparable to ethanol production of 115.8±0.6 g L(-1) (87.1±2.7% of the theoretical ethanol yield) obtained at 5th batch fermentation with 3 g L(-1) yeast extract and 6 g L(-1) polypeptone. Increase of cell density in the concentrated sweet sorghum juice was observed during sequential batch fermentation, as indicated by increased OD600. Utilization of sweet sorghum juice as the sole source, membrane separation, and S. cerevisiae was a cost-effective process for high ethanol production.
采用膜分离（超滤渗透和纳滤浓缩）浓缩甜高粱汁，进行序批式分批发酵，以提高糖含量。酵母 Saccharomyces cerevisiae BY4741 在第 5 批发酵时，从 270.0±22.6 g/L 的糖中获得了 113.7±3.1 g/L 的乙醇（89.1±2.2%的理论乙醇产率），相当于第 1 批发酵时达到的乙醇浓度的 98.7%。该浓度与第 5 批发酵时添加 3 g/L 酵母提取物和 6 g/L 多肽时的乙醇产量 115.8±0.6 g/L（87.1±2.7%的理论乙醇产率）相当。在序批式分批发酵过程中，浓缩甜高粱汁中的细胞密度增加，如 OD600 增加所示。利用甜高粱汁作为唯一来源、膜分离和酿酒酵母是一种具有成本效益的高乙醇生产方法。
