采用超高糖度技术从甜高粱汁中生产乙醇：碳源和氮源补充的影响
Ethanol production from sweet sorghum juice using very high gravity technology: effects of carbon and nitrogen supplementations.
作者信息
Laopaiboon Lakkana, Nuanpeng Sunan, Srinophakun Penjit, Klanrit Preekamol, Laopaiboon Pattana
机构信息
Department of Biotechnology, Faculty of Technology, Khon Kaen University, 123 Mittraparp Road, Khon Kaen 40002, Thailand.
出版信息
Bioresour Technol. 2009 Sep;100(18):4176-82. doi: 10.1016/j.biortech.2009.03.046. Epub 2009 Apr 17.
Ethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP01 was investigated under very high gravity (VHG) fermentation and various carbon adjuncts and nitrogen sources. When sucrose was used as an adjunct, the sweet sorghum juice containing total sugar of 280 g l(-1), 3 g yeast extract l(-1) and 5 g peptone l(-1) gave the maximum ethanol production efficiency with concentration, productivity and yield of 120.68+/-0.54 g l(-1), 2.01+/-0.01 g l(-1) h(-1) and 0.51+/-0.00 g g(-1), respectively. When sugarcane molasses was used as an adjunct, the juice under the same conditions gave the maximum ethanol concentration, productivity and yield with the values of 109.34+/-0.78 g l(-1), 1.52+/-0.01 g l(-1) h(-1) and 0.45+/-0.01 g g(-1), respectively. In addition, ammonium sulphate was not suitable for use as a nitrogen supplement in the sweet sorghum juice for ethanol production since it caused the reduction in ethanol concentration and yield for approximately 14% when compared to those of the unsupplemented juices.
在超高浓度（VHG）发酵以及不同碳源添加剂和氮源条件下，研究了酿酒酵母NP01利用甜高粱汁生产乙醇的情况。当使用蔗糖作为添加剂时，总糖含量为280 g l⁻¹的甜高粱汁、3 g酵母提取物l⁻¹和5 g蛋白胨l⁻¹，乙醇生产效率最高，乙醇浓度、生产率和产率分别为120.68±0.54 g l⁻¹、2.01±0.01 g l⁻¹ h⁻¹和0.51±0.00 g g⁻¹。当使用甘蔗 molasses作为添加剂时，相同条件下的汁液乙醇浓度、生产率和产率最高，分别为109.34±0.78 g l⁻¹、1.52±0.01 g l⁻¹ h⁻¹和0.45±0.01 g g⁻¹。此外，硫酸铵不适合用作甜高粱汁生产乙醇的氮补充剂，因为与未添加氮源的汁液相比，它会使乙醇浓度和产率降低约14%。
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