Fopp-Bayat D, Ocalewicz K
Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Marine Biology and Ecology, Institute of Oceanography, University of Gdansk, Gdynia, Poland.
Reprod Domest Anim. 2015 Aug;50(4):554-9. doi: 10.1111/rda.12521. Epub 2015 Apr 10.
Meiotic gynogenesis was induced in the albino form of sterlet Acipenser ruthenus by activation of eggs with UV-irradiated bester (Huso huso x Acipenser ruthenus) spermatozoa followed by inhibition of the second meiotic division performed by a heat shock. Obtained putative gynogenetic progeny were all albinos. The genetic verification based on three microsatellite DNA markers confirmed the only maternal inheritance of the progeny from the gynogenetic experimental groups. Cytogenetic analysis proved the gynogenetic sterlets were diploids. Application of the albino phenotype together with the molecular and the cytogenetic diagnostic approaches enabled to evaluate the efficiency of the spermatozoa irradiation and application of the heat shock to restore diploid state in the gynogenetic zygotes.
通过用紫外线照射的贝斯特（Huso huso×Acipenser ruthenus）精子激活鱼卵，随后通过热休克抑制第二次减数分裂，在俄罗斯鲟白化变种中诱导减数雌核发育。获得的假定雌核发育后代均为白化个体。基于三个微卫星DNA标记的遗传验证证实了雌核发育实验组后代仅具有母系遗传。细胞遗传学分析证明雌核发育的俄罗斯鲟为二倍体。白化表型与分子和细胞遗传学诊断方法的应用能够评估精子照射的效率以及热休克在恢复雌核发育受精卵二倍体状态中的应用效果。
