Department of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland.
Int J Mol Sci. 2022 Jan 1;23(1):494. doi: 10.3390/ijms23010494.
The objective of the present research was to study the effect of cold shock (3 °C and 6 °C) on fertilized eggs of the sterlet, L. Cold shock was applied for various durations (30, 60 and 90 min) and the ploidy levels, survival, and genotypes of the treated embryos/larvae were recorded. Analysis of ploidy levels confirmed the presence of diploid, triploid, and mosaic (1n/2n, 2n/3n, and 1n/2n/3n) genotypes in experimental groups, while it was strictly diploid in control groups. Microsatellite genotyping confirmed both the incidence of polyspermy and retention of the 2nd polar body in experimental groups. However, patterns of inheritance in all diploid offspring in experimental and control groups revealed classical Mendelian disomic inheritance. Interestingly, the observed mosaic sterlets had normal morphology and were alive. However, some larvae had abnormal morphology which may be due to haploid syndrome. In all treatment groups (treatments: 3 °C-30 min; 3 °C-60 min; 3 °C-90 min; 6 °C-60 min), where the percentage of polyploid/mosaic larvae were high, the mortality was also high. Whereas, in the control groups (where there were only diploid (2n) larvae), the mortality was relatively low.
本研究旨在研究冷休克（3°C 和 6°C）对欧洲小体鲟受精卵的影响。冷休克的处理时间分别为 30、60 和 90 分钟，记录了处理后胚胎/幼鱼的倍性水平、存活率和基因型。倍性水平分析证实了实验各组中存在二倍体、三倍体和嵌合体（1n/2n、2n/3n 和 1n/2n/3n）基因型，而对照组中严格为二倍体。微卫星基因分型证实了实验各组中多精受精和保留第二极体的发生率。然而，实验和对照组所有二倍体后代的遗传模式均显示出经典的孟德尔二体遗传。有趣的是，观察到的嵌合体欧洲小体鲟具有正常的形态，且存活。然而，一些幼鱼具有异常的形态，这可能是由于单倍体综合征。在所有处理组（处理：3°C-30 分钟；3°C-60 分钟；3°C-90 分钟；6°C-60 分钟）中，多倍体/嵌合体幼鱼的比例较高，死亡率也较高。而在对照组（仅有二倍体（2n）幼鱼）中，死亡率相对较低。
Zotero 插件
