Hori Y, Takamoto T, Nishida H, Ishizaki T, Yokoyama M M, Nomura G
Nihon Jinzo Gakkai Shi. 1989 Jul;31(7):723-33.
In order to clarify the abnormalities of cell-mediated immunity in patients with mesangial proliferative glomerulonephritis [IgA nephropathy (IgA N) and Non-IgA nephropathy (Non-IgA N)], lymphocyte subsets were analysed by using monoclonal antibodies with flow cytometric two-color analysis and interleukin-2 (IL-2) production from lymphocytes of the patients was measured by ELISA system. A markedly decreased percentage (11.6 +/- 10.5%) of CD4+ 45R+ cells was found in the patients with IgA N when the results were compared with the normal controls (20 +/- 6%) (P less than 0.01). No difference was found between patients with IgA N and the controls as to the percentage of CD4+ 45R-, CD8+, 11+ and CD8+ 11- cells, respectively. Patients with Non-IgA N also showed a significantly decreased percentage of CD4+ 45R+ cells (10.9 +/- 6.5%), while CD8+ 11+ cells was parallely lowered (7.4 +/- 5%) in compared with the controls (10 +/- 4%). The percentage of HLA-DR positive cells was found to be increased in the patients with both IgA N and Non-IgA N, although the antigen bearing cells were reduced after stimulation with Concanavalin-A (Con-A). No difference in IL-2 production from lymphocyte of both patients groups and the controls cultured with Con-A was found. These results suggested that a deficiency of suppressor inducer T cells played a part of the pathogenesis of IgA N and Non-IgA N.
为了阐明系膜增生性肾小球肾炎[IgA肾病（IgAN）和非IgA肾病（Non-IgAN）]患者细胞介导免疫的异常情况，采用单克隆抗体通过流式细胞仪双色分析来检测淋巴细胞亚群，并采用ELISA系统检测患者淋巴细胞产生白细胞介素-2（IL-2）的情况。与正常对照组（20±6%）相比，IgAN患者中CD4+45R+细胞的百分比显著降低（11.6±10.5%）（P<0.01）。IgAN患者与对照组在CD4+45R-、CD8+、11+和CD8+11-细胞的百分比方面分别未发现差异。Non-IgAN患者也显示CD4+45R+细胞的百分比显著降低（10.9±6.5%），而与对照组（10±4%）相比，CD8+11+细胞也相应减少（7.4±5%）。发现IgAN和Non-IgAN患者中HLA-DR阳性细胞的百分比均增加，尽管用刀豆蛋白A（Con-A）刺激后抗原呈递细胞减少。两组患者和用Con-A培养的对照组淋巴细胞产生IL-2的情况未发现差异。这些结果表明，抑制诱导性T细胞缺陷在IgAN和Non-IgAN的发病机制中起一定作用。
