Pokudin N I, Orlov S N, Kotelevtsev Iu V, Postnov Iu V
Kardiologiia. 1989 Aug;29(8):83-8.
The erythrocytes from two spontaneously hypertensive rat strains (SHR and MHS) differ from those of control strains such as WKY and MNS in showing lower values of the mean volume and higher Na,K-cotransport rates. After 4-hour incubation in the presence of orthovanadate 45Ca levels in the erythrocytes from SHR was twice as high as in those from WKY. Under the same conditions, hemoglobin crystallization occurred, this process in SHR erythrocytes took place more intensively. No difference was found in these parameters between MNS and MHS. The fractions enriched with young and old cells remained to display variations in the mean volume of cells, Na,K-cotransport rates, 45Ca levels and hemoglobin crystallization, which were recorded between SHR and WKY. The WKY-SHR hybrids of second generation indicated that only Na,K-cotransport rates of the parameters examined positively correlated with blood pressures. From the findings it was concluded that the differences in the volume of erythrocytes and their permeability for cations were not due to the changed time course of cell aging in the blood bed, but to the specific characteristics of cytoskeleton protein arrangement, which were primarily typical of a rat reticulocytic membrane.
两种自发性高血压大鼠品系（SHR和MHS）的红细胞与对照品系如WKY和MNS的红细胞不同，表现为平均体积值较低和钠钾协同转运速率较高。在原钒酸盐存在下孵育4小时后，SHR红细胞中的45Ca水平是WKY红细胞的两倍。在相同条件下，血红蛋白发生结晶，SHR红细胞中的这一过程更为强烈。MNS和MHS在这些参数上没有差异。富含年轻和衰老细胞的组分在细胞平均体积、钠钾协同转运速率、45Ca水平和血红蛋白结晶方面仍表现出SHR和WKY之间记录到的差异。第二代WKY-SHR杂交种表明，在所检测的参数中，只有钠钾协同转运速率与血压呈正相关。从这些发现得出结论，红细胞体积及其对阳离子的通透性差异不是由于血床中细胞衰老时间进程的改变，而是由于细胞骨架蛋白排列的特定特征，这主要是大鼠网织红细胞膜的典型特征。
