Steudler Susanne, Bley Thomas
Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, Bergstraße 120, 01069, Dresden, Germany,
Adv Biochem Eng Biotechnol. 2015;149:223-52. doi: 10.1007/10_2014_300.
Filamentous fungi, especially basidiomycetes, produce a wide range of metabolites, many of which have potential biotechnological and industrial applications. Solid-state fermentation (SSF) is very suitable for the cultivation of basidiomycetes since it mimics the natural habitat of these fungi. Some of the major advantages of SSF are the robustness of the process, the use of low-cost residual materials as substrates, and the reduced usage of water. However, monitoring key variables is difficult, which makes process control a challenge. Specifically, it is very difficult to determine the biomass during SSF process involving basidiomycetes. This is problematic, as the biomass is normally a key variable in mass and energy balance equations. Further, the success of fungal SSF processes is often evaluated, in part, based on the growth of the fungus. Direct determination of the dry weight of biomass is impossible and indirect quantification techniques must be used. Over the years, various determination techniques have been developed for the quantification of fungal biomass in SSF processes. The current review gives an overview of various direct and indirect biomass determination methods, discussing their advantages and disadvantages.
丝状真菌，尤其是担子菌，能产生多种代谢产物，其中许多具有潜在的生物技术和工业应用价值。固态发酵（SSF）非常适合担子菌的培养，因为它模拟了这些真菌的自然栖息地。固态发酵的一些主要优点包括工艺的稳健性、使用低成本的残余材料作为底物以及减少水的使用。然而，监测关键变量很困难，这使得过程控制成为一项挑战。具体而言，在涉及担子菌的固态发酵过程中确定生物量非常困难。这是个问题，因为生物量通常是质量和能量平衡方程中的关键变量。此外，真菌固态发酵过程的成功与否通常部分基于真菌的生长情况来评估。直接测定生物量的干重是不可能的，必须使用间接定量技术。多年来，已经开发了各种测定技术来定量固态发酵过程中的真菌生物量。本综述概述了各种直接和间接生物量测定方法，并讨论了它们的优缺点。
